Abstract
Techniques to trace and isolate brown adipocyte precursor and adipocytes during development and disease are essential to fully understand brown adipose tissue development and function. Here we report several protocols using the R26R-mTmG reporter mice in thermogenic tissues based on confocal microscopy and fluorescence based flow cytometry. These techniques may be useful to understand the influence of genetic or environmental alterations in brown adipocyte precursors and adipocyte biology.
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Acknowledgements
We would like to thank the Sanchez-Gurmaches lab for valuable discussions. We thank Dr. John Matthew Kofron and the Confocal Imaging Core at Cincinnati Children’s Hospital Medical Center for sharing their technical expertise. This work was supported by grants from the American Heart Association (18CDA34080527) to J.S.-G. and a CCHMC Trustee Award to J.S.-G. R.M. was supported by a postdoctoral fellowship from the American Heart Association (19POST34380545). This project was supported in part by NIH P30 DK078392 (Confocal Imaging Core) of the Digestive Diseases Research Core Center in Cincinnati.
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Mukherjee, R., Sanchez-Gurmaches, J. (2022). Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice. In: Guertin, D.A., Wolfrum, C. (eds) Brown Adipose Tissue. Methods in Molecular Biology, vol 2448. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2087-8_13
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DOI: https://doi.org/10.1007/978-1-0716-2087-8_13
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