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Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

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Bacterial Virulence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2427))

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Abstract

Group A streptococcus (GAS) necrotizing fasciitis (NF) causes high morbidity and mortality despite prompt intravenous administration of antibiotics, surgical soft-tissue debridement, and supportive treatment in the intensive care unit. Since there is no effective vaccine against GAS infections, a comprehensive understanding of NF pathogenesis is required to design more efficient treatments. To increase our understanding of NF pathogenesis, we need a reliable animal model that mirrors, at least in part, the infectious process in humans. This chapter describes a reliable murine model of human NF that mimics the histopathology observed in humans, namely the destruction of soft tissue, a paucity of infiltrating neutrophils, and the presence of many gram-positive cocci at the center of the infection.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, The Institute for Medical Research, Israel-Canada (IMRIC), Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

    Miriam Ravins, Yael Kaufman, Aparna Anand, Abhinay Sharma & Emanuel Hanski

  2. Singapore-HUJ Alliance for Research and Enterprise, MMID Phase II, Campus for Research Excellence and Technological Enterprise (CREATE), Singapore, Singapore

    Poornima Ambalavanan, Debabrata Biswas, Rachel Ying Min Tan, Kimberly Xuan Zhen Lim & Emanuel Hanski

  3. Department of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Poornima Ambalavanan, Debabrata Biswas, Rachel Ying Min Tan, Kimberly Xuan Zhen Lim & Emanuel Hanski

Authors
  1. Miriam Ravins
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  2. Poornima Ambalavanan
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  3. Debabrata Biswas
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  4. Rachel Ying Min Tan
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  5. Kimberly Xuan Zhen Lim
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  6. Yael Kaufman
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  7. Aparna Anand
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  8. Abhinay Sharma
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  9. Emanuel Hanski
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Corresponding author

Correspondence to Emanuel Hanski .

Editor information

Editors and Affiliations

  1. The Infectious Diseases Research Laboratory, Sheba Medical Center, Tel-Hashomer, Israel

    Ohad Gal-Mor

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Ravins, M. et al. (2022). Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis. In: Gal-Mor, O. (eds) Bacterial Virulence. Methods in Molecular Biology, vol 2427. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1971-1_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1971-1_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1970-4

  • Online ISBN: 978-1-0716-1971-1

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