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A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons

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Cancer Biomarkers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2413))

Abstract

Pain associated with chemotherapy and radiation therapy is one of the most common reasons for discontinuation of these treatments and has a dramatic effect on the quality of life in cancer patients. However, the mechanisms underlying chemotherapy and radiation therapy associated with pain are not well understood. Pain sensations are mediated through sensory neurons whose cell bodies are located in the dorsal root ganglia (DRG). Pain mediators activate these sensory neurons causing an influx of ions, including calcium. One common technique to study pain is to use primary cell culturing mouse DRG to study this calcium influx in vitro. This protocol details from an isolation to culture and maintenance of DRG neurons and functional recording using calcium imaging caused by either pain mediators or neuronal sensitization that are induced by drugs that are often used in the treatment of cancer.

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Acknowledgments

This work was supported by start-up fund awarded to SKM.

Author information

Author notes

  1. Santosh K. Mishra

    Present address: Department of Molecular Biomedical Sciences, North Carolina State University College of Veterinary Medicine, Raleigh, NC, USA

Authors and Affiliations

  1. Department of Molecular Biomedical Sciences, North Carolina State University College of Veterinary Medicine, Raleigh, NC, USA

    Joshua J. Wheeler

  2. Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA

    Joshua J. Wheeler & Santosh K. Mishra

  3. Department of Psychology, University of Chicago, Chicago, IL, USA

    John M. Davis

  4. Comparative Pain Research and Education Center, North Carolina State University, Raleigh, NC, USA

    Santosh K. Mishra

Authors
  1. Joshua J. Wheeler
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  2. John M. Davis
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  3. Santosh K. Mishra
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Corresponding author

Correspondence to Santosh K. Mishra .

Editor information

Editors and Affiliations

  1. Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, USA

    Gagan Deep

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Wheeler, J.J., Davis, J.M., Mishra, S.K. (2022). A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons. In: Deep, G. (eds) Cancer Biomarkers. Methods in Molecular Biology, vol 2413. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1896-7_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1896-7_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1895-0

  • Online ISBN: 978-1-0716-1896-7

  • eBook Packages: Springer Protocols

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