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Design of Replication-Competent VSV- and Ervebo-Vectored Vaccines Against SARS-CoV-2

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2410))

Abstract

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a global public health emergency. Several vaccine candidates have been developed in response to the COVID-19 pandemic. One approach is to construct live-recombinant viruses expressing the SARS-CoV-2 spike protein (S) as vaccine candidates. The vesicular stomatitis virus (VSV) vector is a mature vaccine platform which was successfully developed as a vaccine against Ebola virus (EBOV), leading to its licensure by the Food and Drug Administration (FDA) in December 2019. Based on this work, we developed two live, replication-competent VSV-vectored vaccines against SARS-CoV-2: (1) a VSV expressing the S protein of SARS-CoV-2 and (2) a bivalent VSV expressing the S protein of SARS-CoV-2 and the glycoprotein (GP) of EBOV. This protocol describes the methodologies for the design, cloning, rescue, and preparation of these recombinant VSV vaccines.

Qixing Liu and Zhe Ding are co-first authors.

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Author information

Authors and Affiliations

  1. Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China

    Qixing Liu, Zhe Ding, Jiaming Lan & Gary Wong

  2. Département de microbiologie-infectiologie et d’immunologie, Université Laval, Québec, QC, Canada

    Gary Wong

Authors
  1. Qixing Liu
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  2. Zhe Ding
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  3. Jiaming Lan
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  4. Gary Wong
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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Liu, Q., Ding, Z., Lan, J., Wong, G. (2022). Design of Replication-Competent VSV- and Ervebo-Vectored Vaccines Against SARS-CoV-2. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2410. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1884-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1884-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1883-7

  • Online ISBN: 978-1-0716-1884-4

  • eBook Packages: Springer Protocols

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