Abstract
The immune response in the liver is a highly dynamic process involving the recruitment of many types of immune cells. As a powerful imaging technique, intravital microscopy has been widely used for real-time observation and quantification of cell movements in living animals. Here we describe the use of an in vivo half-dissociated preparation method combined with intravital confocal microscopy to observe the dynamic activities of invariant natural killer T cells in the liver of CXCR6GFP/+ transgenic mice. We believe that this method will enable researchers to explore the dynamics of many other types of immune cells in the liver.
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References
Bendelac A, Lantz O, Quimby ME, Yewdell JW, Bennink JR, Brutkiewicz RR (1995) CD1 recognition by mice NK1+ T lymphocytes. Science 268:863–865
Gapin L (2016) Development of invariant natural killer T cells. Curr Opin Immunol 39:68–74
Matsuda JL, Mallevaey T, Scott-Browne J et al (2008) CD1d-restricted iNKT cells, the “Swiss-Army knife” of the immune system. Curr Opin Immunol 20:358–368
Bendelac A, Savage PB, Teyton L (2007) The biology of NKT cells. Annu Rev Immunol 25:297–336
Chawla A, Nguyen KD, Goh YP (2011) Macrophage-mediated inflammation in metabolic disease. Nat Rev Immunol 11:738–749
Kohlgruber AC, Donado CA, LaMarche NM et al (2016) Activation strategies for invariant natural killer Tcells. Immunogenetics 68:649–663
Acknowledgments
This work was supported by the National Natural Science Foundation of China [grant numbers 81871241 and 81571614].
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© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Hong, Z., Zhutian, Z., Fei, W. (2021). Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice. In: Liu, C. (eds) Invariant Natural Killer T-Cells. Methods in Molecular Biology, vol 2388. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1775-5_14
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DOI: https://doi.org/10.1007/978-1-0716-1775-5_14
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1774-8
Online ISBN: 978-1-0716-1775-5
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