Abstract
In the last decades, the increasing rate of multidrug-resistant bacteria to classical antibiotics has driven research towards identification of other means to fight bacterial infections. In this context, intracellular and/or invasive facultative intracellular bacteria represent a particular problem as common antimicrobials are often not able to reach an effective intracellular concentration. In this regard, cell-penetrating peptides (CPP) can mediate the internalization of previously nonpermeable antimicrobial compounds into the cytoplasm of host cells where they efficiently kill intracellular pathogens. This chapter describes the conjugation of CPPs with antimicrobial agents for the delivery into infected cells. Furthermore, different antimicrobial activity assays will be described including the CPP-mediated delivery of an antimicrobial agent for the treatment of intracellular infections.
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Acknowledgments
This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG) (CRC1009 TP B03) and NIH (RO1 1R56AR072594-01A1). I thank A.-S. Stolle for critical reading of the manuscript and I like to thank all the past and present members of the Institute of Infectiology—ZMBE for their valuable contributions, particularly M. Gomarasca and T.F.C. Martins for establishing the protocols.
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Rüter, C. (2022). Delivery of Antibiotics by Cell-Penetrating Peptides to Kill Intracellular Pathogens. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_22
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DOI: https://doi.org/10.1007/978-1-0716-1752-6_22
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