Abstract
Cancer is the world’s second major cause of death, and despite significant advances in drug distribution, bacteria-based therapies still need to be developed. Bacterial cell free supernatant (CFS) remedies have many advantages relative to the use of traditional therapeutic agents to mitigate the side effects caused by therapies. The objective of the protocol was to discover the possible cytotoxicity of CFS, ethanol extract and its derived fractions (chloroform, ethyl acetate, butanol, and aqueous). DNA fragmentation assay methodology was applied to determine the anticancer activity in human cell lines (NCI-H460, HepG2).
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Madar, I., Sultan, G., Chelliah, R., Oh, DH. (2022). Screening for Anticancer Activity: DNA Fragmentation Assay. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_58
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_58
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1727-4
Online ISBN: 978-1-0716-1728-1
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