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Isolation and Screening of Phytase Producing Microorganisms: An Essential Bioinput for Soil Fertility

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Practical Handbook on Agricultural Microbiology

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Abstract

Phytase is one of the identified enzymes which plays a vital role in phosphorous (P) cycle by mobilization of immobilized P from plant material. Although there is various phosphate solubilizing biofertilizer agents reported, they are majorly targeted for mobilizing rock phosphates in soil. The Phosphate solubilizing biofertilizers are required for mobilizing the immobilized form of P from plant source i.e., from phytate. The protocol is based on the principle that specific phytate agar medium contains calcium/sodium phytate, which is utilized as P source by phytase producing microorganism indicated by zone of clearance around the colony. The further quantification is executed by measuring the free phosphate by spectrophotometric method.

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References

  1. Khan AA, Jilani G, Akhtar MS et al (2009) Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J Agric Biol Sci 1:48–58

    Google Scholar 

  2. Raboy V, Dickinson DB (1987) The timing and rate of phytic acid accumulation in develo** soybean seeds. Plant Physiol 85(3):841–844

    Article  CAS  Google Scholar 

  3. Mohite BV, Koli SH, Borase HP et al (2019) New edge agricultural bioinputs. In: Singh P, Gupta VK, Prabha R (eds) Microbial interventions in agriculture and environment. Springer, Singapore, pp 353–380

    Chapter  Google Scholar 

  4. Jorquera M, Martinez O, Maruyama F (2008) Current and future biotechnological applications of bacterial phytases and phytase-producing bacteria. Microbes Environ 23:182–191

    Article  Google Scholar 

  5. Gibson DM (1987) Production of extracellular phytase from Aspergillus ficuum on starch media. Biotechnol Lett 9:305–310

    Article  CAS  Google Scholar 

  6. Choi YM, Suh HJ, Kim JM (2001) Purification and properties of extracellular phytase from Bacillus sp. KHU-10. J Protein Chem 20:287–292

    Article  CAS  Google Scholar 

  7. Bae HD, Yanke LJ, Cheng K-J, Selinger LB (1999) A novel staining method for detecting phytase activity. J Microbiol Methods 39:17–22

    Article  CAS  Google Scholar 

  8. Pandey A, Szakacs G, Soccol CR et al (2001) Production, purification and properties of microbial phytases. Bioresour Technol 77(3):203–214

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Microbiology, Bajaj College of Science, Wardha, Maharashtra, India

    Bhavana V. Mohite

  2. School of Life Sciences, KBC North Maharashtra University, Jalgaon, Maharashtra, India

    Kiran Marathe, Narendra Salunkhe & Satish V. Patil

Authors
  1. Bhavana V. Mohite
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  2. Kiran Marathe
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  3. Narendra Salunkhe
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  4. Satish V. Patil
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Editor information

Editors and Affiliations

  1. Uka Tarsadia University, Surat, Gujarat, India

    Natarajan Amaresan

  2. Uka Tarsadia University, Surat, Gujarat, India

    Prittesh Patel

  3. Uka Tarsadia University, Surat, Gujarat, India

    Dhruti Amin

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mohite, B.V., Marathe, K., Salunkhe, N., Patil, S.V. (2022). Isolation and Screening of Phytase Producing Microorganisms: An Essential Bioinput for Soil Fertility. In: Amaresan, N., Patel, P., Amin, D. (eds) Practical Handbook on Agricultural Microbiology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1724-3_45

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  • DOI: https://doi.org/10.1007/978-1-0716-1724-3_45

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1723-6

  • Online ISBN: 978-1-0716-1724-3

  • eBook Packages: Springer Protocols

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