Abstract
In this chapter, a detailed preparation of W/O emulsions using microfluidic devices is presented. Soybean oil is proposed as continuous phase, and two hydrophilic polymers, sodium caseinate and alginate, are proposed as dispersed phases. This approach enables the controlled encapsulation of different cells and/or bioactive compounds. The encapsulation of the probiotic strain Lactiplantibacillus plantarum CIDCA 83114 is presented.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Francesko A, Cardoso VF, Lanceros-Mendez S (2019) Lab-on-a-chip technology and microfluidics. In: Santos HA, Liu D, Zhag H (eds) Microfluidics for pharmaceutical applications from nano/micro systems fabrication to controlled drug delivery. Elsevier, Amsterdam, pp 3–36
Utada AS, Lorenceau E, Link DR et al (2005) Monodisperse double emulsions generated from a microcapillary device. Science 308:537–541. https://doi.org/10.1126/science.1109164
Chu LY, Utada AS, Shah RK et al (2007) Controllable monodisperse multiple emulsions. Angew Chem Int Ed 46:8970–8974. https://doi.org/10.1002/anie.200701358
Vinner GK, Vladisavljević GT, Clokie MR et al (2017) Microencapsulation of Clostridium difficile specific bacteriophages using microfluidic glass capillary devices for colon delivery using pH triggered release. PLoS One 12:e0186239. https://doi.org/10.1371/journal.pone.0186239
Bazban-Shotorbani S, Dashtimoghadam E, Karkhaneh A et al (2016) Microfluidic directed synthesis of alginate nanogels with tunable pore size for efficient protein delivery. Langmuir 32:4996–5003. https://doi.org/10.1021/acs.langmuir.5b04645
Niu X, de Mello AJ (2012) Building droplet-based microfluidic systems for biological analysis. Biochem Soc Trans 40:615–623. https://doi.org/10.1042/BST20120005
Garrote GL, Abraham AG, De Antoni GL (2001) Chemical and microbiological characterisation of kefir grains. J Dairy Res 68:639–652. https://doi.org/10.1017/S0022029901005210
de Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x
Zinchenko A, Devenish SRA, Kintses B et al (2014) One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution. Anal Chem 86:2526–2533. https://doi.org/10.1021/AC403585P
Martin-Dejardin F, Ebel B, Lemetais G et al (2013) A way to follow the viability of encapsulated Bifidobacterium bifidum subjected to a freeze-drying process in order to target the colon: interest of flow cytometry. Eur J Pharm Sci 49:166–174. https://doi.org/10.1016/j.ejps.2013.02.015
Acknowledgments
This work was supported by the Argentinean Agency for the Scientific and Technological Promotion (ANPCyT) [Projects PICT(2017)/1344 and PICT start-up (2016)/4808. G.Q. is fellow from CONICET. E.G. and A.G.-Z. are members of the research career CONICET.
Competing Interests: The authors declare that they have no competing interests.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this chapter
Cite this chapter
Quintana, G., Gerbino, E., Gómez-Zavaglia, A. (2021). Microfluidic Glass Capillary Devices: An Innovative Tool to Encapsulate Lactiplantibacillus plantarum. In: Gomez-Zavaglia, A. (eds) Basic Protocols in Encapsulation of Food Ingredients. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1649-9_7
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1649-9_7
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1648-2
Online ISBN: 978-1-0716-1649-9
eBook Packages: Springer Protocols