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Methods Used to Make Lipid Nanoparticles to Deliver LNA Gapmers Against lncRNAs into Acute Myeloid Leukemia (AML) Blasts

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Long Non-Coding RNAs in Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2348))

Abstract

Develo** strategies to target lncRNAs are needed. In this chapter, we describe in detail a method to deliver antisense oligonucleotides into acute myeloid leukemia cells using lipid nanoparticles tagged with the transferrin receptor. While this chapter is focused on the delivery method, we also discuss important considerations about the design of antisense oligonucleotides (ASOs). The strategy described here has been used successfully to deliver ASOs into leukemic blasts and stem cells.

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Change history

  • 06 October 2021

    In the original version of this book, Chapter 11 was published with incorrect author name and affiliation. It has now been updated in the revised version of this book.

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Author information

Authors and Affiliations

  1. Division of Pharmaceutics and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, USA

    Chun-Tien Kuo & Robert J. Lee

  2. Division of Hematology, Department of Medicine, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    Ramiro Garzon

Authors
  1. Chun-Tien Kuo
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  2. Robert J. Lee
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  3. Ramiro Garzon
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Corresponding author

Correspondence to Ramiro Garzon .

Editor information

Editors and Affiliations

  1. Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain

    Alfons Navarro

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© 2021 Springer Science+Business Media, LLC, part of Springer Nature

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Kuo, CT., Lee, R.J., Garzon, R. (2021). Methods Used to Make Lipid Nanoparticles to Deliver LNA Gapmers Against lncRNAs into Acute Myeloid Leukemia (AML) Blasts. In: Navarro, A. (eds) Long Non-Coding RNAs in Cancer. Methods in Molecular Biology, vol 2348. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1581-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1581-2_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1580-5

  • Online ISBN: 978-1-0716-1581-2

  • eBook Packages: Springer Protocols

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