Abstract
Lymph node invasion by tumor cells is an important process in the progression of melanoma and is a poor prognostic factor for patients with this cancer. Before they are able to spread to regional lymph nodes, though, melanoma cells must first adhere to lymphatic endothelium and transmigrate into the lymphatic vasculature. In order to study melanoma cell adhesion to lymphatic endothelial cells and the factors that regulate this process, we have developed an in vitro flow cytometry-based assay to measure melanoma cell attachment to lymphatic endothelial cells. This assay will be a useful tool for investigating the interactions that take place between melanoma cells and lymphatic endothelial cells during the adhesion process.
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Acknowledgments
D5.1G4 murine melanoma cells were a generous gift of Dr. Jerry Neiderkorn (University of Texas Southwestern Medical School). The SV-LEC murine melanoma cell line was kindly provided by Dr. Jonathan Alexander (Louisiana State University Health Sciences Center). This work was supported by funding from Virginia’s Commonwealth Health Research Board and a Jeffress Trust Awards Program in Interdisciplinary Research Grant from the Thomas F. and Kate Miller Jeffress Memorial Trust (Bank of America, N.A., Trustee) to KMH. We also thank Mr. Michael Hargadon and Mrs. Patricia Hargadon for generous donations to support the involvement of Hampden-Sydney College undergraduate students in this research.
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Hargadon, K.M., Johnson, C.E. (2021). A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_10
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DOI: https://doi.org/10.1007/978-1-0716-1205-7_10
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