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Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes

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Structural Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2199))

Abstract

The scattering profiles at small angles, obtained after an X-ray beam is incident on biological samples (protein), are nowadays successfully used to obtain important structural information. Small angle X-ray scattering (SAXS) is now helpful in providing information about shape, conformation, and assembly state of molecules, besides macromolecular folding–unfolding, aggregation, and extended conformations. The article discusses here a protocol to identify those fractions of heterogeneous proteins that are rich in homogeneous samples, testified by proper conformation and protein activity. The protocol in reference to a class of proteins known as metal binding (transporter) proteins or ion channels is discussed using applications of SAXS and metal radioisotopes. With requisite modifications, the protocol can be adapted to other classes of proteins.

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Author information

Authors and Affiliations

  1. Department of Infection Biology, London School of Hygiene & Tropical Medicine, London, UK

    Shah Kamranur Rahman

Authors
  1. Shah Kamranur Rahman
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Correspondence to Shah Kamranur Rahman .

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Editors and Affiliations

  1. Department of Applied Biology and Chemical Technology and the State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hung Hom, Hong Kong

    Yu Wai Chen

  2. BMI Biotechnology Appraisals & Investments Limited, Wan Chai, Hong Kong

    Chin-Pang Bennu Yiu

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Rahman, S.K. (2021). Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes. In: Chen, Y.W., Yiu, CP.B. (eds) Structural Genomics. Methods in Molecular Biology, vol 2199. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0892-0_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0892-0_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0891-3

  • Online ISBN: 978-1-0716-0892-0

  • eBook Packages: Springer Protocols

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