Abstract
Bioprinting has emerged as a promising method for precise spatiotemporal patterning of biological materials such as living cells, genetic materials, and proteins, which are sensitive to any other fabrication techniques. Bioprinting allows the generation of tissue constructs and models that closely mimic the anatomical and physiological attributes of a chosen tissue. In vitro toxicology assays can greatly benefit from bioprinting as drugs can be screened with higher efficiencies in a significantly reduced period. This protocol describes a method for fabricating bioprinted cartilage constructs which can be used for in vitro toxicology studies employing a scalable “tissue strand” bioprinting modality.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Dababneh AB, Ozbolat IT (2014) Bioprinting technology: a current state-of-the-art review. J Manuf Sci Eng 136:61016. https://doi.org/10.1115/1.4028512
Ozbolat IT (2015) Scaffold-based or scaffold-free bioprinting: competing or complementing approaches? J Nanotechnol Eng Med 6:24701. https://doi.org/10.1115/1.4030414
Wu Y, Wong YS, Fuh JYH (2017) Degradation behaviors of geometric cues and mechanical properties in a 3D scaffold for tendon repair. J Biomed Mater Res A 105:1138–1149. https://doi.org/10.1002/jbm.a.35966
Yu Y, Moncal KK, Li J et al (2016) Three-dimensional bioprinting using self-assembling scalable scaffold-free “tissue strands” as a new bioink. Sci Rep 6:28714
Ozbolat IT, Hospodiuk M (2016) Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 76:321–343. https://doi.org/10.1016/j.biomaterials.2015.10.076
Ozbolat IT, Moncal KK, Gudapati H (2017) Evaluation of bioprinter technologies. Addit Manuf 13:179–200. https://doi.org/10.1016/j.addma.2016.10.003
Gudapati H, Dey M, Ozbolat I (2016) A comprehensive review on droplet-based bioprinting: Past, present and future. Biomaterials 102:20–42. https://doi.org/10.1016/j.biomaterials.2016.06.012
Murphy SV, Atala A (2014) 3D bioprinting of tissues and organs. Nat Biotechnol 32:773–785
Peng W, Unutmaz D, Ozbolat IT (2016) Bioprinting towards physiologically relevant tissue models for pharmaceutics. Trends Biotechnol 34:722–732. https://doi.org/10.1016/j.tibtech.2016.05.013
Nicholson JK, Connelly J, Lindon JC, Holmes E (2002) Metabonomics: a platform for studying drug toxicity and gene function. Nat Rev Drug Discov 1:153–161. https://doi.org/10.1038/nrd728
Yu Y, Zheng H, Buckwalter JA, Martin JA (2014) Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage. Osteoarthritis Cartilage 22:1318–1326. https://doi.org/10.1002/nbm.3369
Acknowledgments
This work has been supported by National Science Foundation Award # 1624515. Pallab Datta acknowledges the Department of Science and Technology, Government of India, INSPIRE Faculty Award. The author thanks Dr. Weijie Peng (Penn State University) for his fruitful insights and Dr. Adil Akkouch (The University of Iowa) for his assistance with SEM imaging. The authors also thank Dr. James A. Martin (The University of Iowa) for providing facilities with mechanical testing.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Datta, P., Wu, Y., Yu, Y., Moncal, K.K., Ozbolat, I.T. (2021). A Scaffold Free 3D Bioprinted Cartilage Model for In Vitro Toxicology. In: Rainer, A., Moroni, L. (eds) Computer-Aided Tissue Engineering. Methods in Molecular Biology, vol 2147. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0611-7_15
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0611-7_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0610-0
Online ISBN: 978-1-0716-0611-7
eBook Packages: Springer Protocols