Microbiology of Grape Surfaces and its Importance for Wine Quality

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Aerial Plant Surface Microbiology

Abstract

Although humankind has been involved in winemaking since the very beginning of civilization, it was not until the second half of the last century that Pasteur (1857) demonstrated the microbial origin of spontaneous fermentations. Years later, Hansen (1903) established the taxonomic basis for a rational approach to the study of yeasts associated with grapes and to those actually involved in the fermentation process. By the middle of the present century studies in microbial ecology have shown that the vinification process involves a sequential proliferation of different types of yeast and bacteria coming either from the vineyard itself or from the cellar (Mrak and McClung, 1940; Castelli, 1954 and Galzy, 1958)

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References

  • Al-Aidroos, K. and Bussey H. 1977, Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor. Can. J. Microbiol. 24:228–237.

    Google Scholar 

  • Barre, P. 1980, Role du facteur killer dans la concurrence entre souches de levures. Bull. O.I.V. 52:560–567.

    Google Scholar 

  • Barre, P. 1984, Le mecanisme killer dans la concurrence entre souches de levures. Evaluation et prise en compte. Bull. O.I.V. 641:635–643.

    Google Scholar 

  • Belin, J.M. 1972, Reserches sur la repartition des levures a la surface de la grappe de raisin. Vitis 11:135–145.

    Google Scholar 

  • Beech, F.W. and Thomas, S. 1985, Action antimicrobienne de l’anhidride sulfureux. Bull. O.I.V. 58:564–581.

    CAS  Google Scholar 

  • Benda, J. 1970, Natural and controlled microbial processes in grape must in young wine. Bayer Ladwisch. Jahrh. 47:19–29.

    Google Scholar 

  • Benda, J. 1985, Yeast in winemaking-investigating so-called killer yeasts during fermentation. Der Deutsche Weinbau. Wisbaden. 40:166–171.

    Google Scholar 

  • Bevan, E. A. and MacKover, M. 1963, The physiologycal basis of the killer character in yeast. In: Geerts, S.J. (ed.) Proc.Intern.Congress of Genet. Le Hage. 11:127.

    Google Scholar 

  • Bisiach, M., Minervini, G. and Zerbetto, F. 1986, Possible integrated control of grapevine sour rot. Vitis 25:118–128.

    CAS  Google Scholar 

  • Blanco, P., Sieiro, C., Diaz, A. and Villa, T.G. 1994, Production and Partial Characterization of an Endopoly-galacturonase from Saccharomyces cerevisiae. Can. J. Microbiol. 40:974–977.

    Article  PubMed  CAS  Google Scholar 

  • Bouix, M., Leveau, J.Y, et Cuinier C. 1981, Determination de l’origine de levures de vinification par une methode de differenciation fine des souches. Conn. Vigne Vin. 15:41–52.

    Google Scholar 

  • Bruenn, J., Bobek, L., Brennan, V. and Held, W. 1980, Yeast viral RNA polymerase is a transcriptase. Nucleic Acid Res. 8:2985–2997.

    Article  PubMed  CAS  Google Scholar 

  • Bussey, H. 1981, Physiology of killer factor in yeast. Adv. Microbiol. Physiol. 23:93–122.

    Article  Google Scholar 

  • Cansado, J., Velazquez, J.B., Sieiro, C., Calo, P., Longo, E. and Villa, T.G. 1992, Role of killer strains of Saccharomyces cerevisiae in spontaneous fermentations, pp. 127–142 In: Villa, T.G. and Abalde, J. (eds.) Profiles on Biotechnology Santiago University Press. Spain.

    Google Scholar 

  • Cansado, J., Longo, E., Calo, P., Sieiro, C., Velazquez, J.B. and Villa, T.G. 1991, Role of killer character in spontaneous fermentations from NW Spain: ecology, distribution and significance Appl. Microbiol. Biotechnol.34:643–647.

    Article  Google Scholar 

  • Castelli, T. 1954, Les agents de la fermentation vinaire. Arch. Microbiol. 20:323–342.

    CAS  Google Scholar 

  • Ciolfi, G. 1984, Effect of pH on the killer phenotype in Saccharomyces cerevisiae. Riv. Vitic. Enol. 37:76–79.

    Google Scholar 

  • Cuinier, C. 1980, Origine des levures assurant l’elaboration d’un vin blanc de Tourraine. Identification des species. Connaiss. Vigne Vin 14:111–126.

    Google Scholar 

  • Davenport, R.R. 1976, Distribution of yeasts and yeast-like organisms from aerial surfaces of develo** apples and grapes, pp.325–359 In: Dickinson, C.H. and Preecee, T.F. (eds.) Microbiology of Aerial Plant Surfaces, Academic Press. London.

    Google Scholar 

  • dela Peña, P., Barros, F., Gascón, S., Lazo, P.S. and Ramos, S. 1981, Effect of yeast killer toxin on sensitive cells of S. cerevisiae. J. Biol. Chem. 256:10420–10425.

    PubMed  Google Scholar 

  • Domerq, S. 1956, Etude et classification des levures de vin de la Gironde. These de Docteur-Ingenieur. Universite de Bordeaux

    Google Scholar 

  • Esteban, R. and Fujimura, T. 1992, RNA expresion vectors based on the killer dsRNA viruses from Saccharomyces cerevisiae, pp. 33–42 In: Villa, T.G. and Abalde, J. (eds.) Profiles on Biotechnology Santiago University Press, Spain.

    Google Scholar 

  • Farris, G.A., Forteleoni, M. and Fatichenti, F. 1987, Effects microbiologique de la recolte mecanique. Rev. Fr. Oenol. 110:42–45.

    Google Scholar 

  • Federici, F. 1985, Production, purification and partial characterization of an endopolygalacturonase from Cryptococcus albidus var. albidus. Antonie van Leeuwenhoek 51:139–150.

    Article  PubMed  CAS  Google Scholar 

  • Fleet, G.H., Lafon-Lafourcade, S. and Ribereau-Gayon, 1984, Evolution of yeast and lactic acid bacteria during fermentation and storage of Bordeaux wines. Appl. Env. Microbiol. 48:1034–1038.

    CAS  Google Scholar 

  • Fleet, G.H. 1990, Which yeast species really conduct the fermentation?, pp. 153–156 In: Williams, P.I., Davison, D. and Lee, T.H. (eds.) Proceedings of the Seventh Australian Wine Industry, Australian Industrial Publishers, Adelaide, S.A.

    Google Scholar 

  • Fogarty, W.M. and Kelly, C.T. 1983, Pectic enzymes, pp. 131–182 In: Fogarty, W.M. (ed.) Microbial Enzymes and Biotechnology, Applied Science Publishers, London.

    Google Scholar 

  • Frezier, V. and Dubourdieu, D. 1992, Ecology of yeast strain S. cerevisiae during a spontaneous fermentation in a Bordeaux winery. Am. J. Enol. Vitic. 43:375–380.

    Google Scholar 

  • Fujimura, T., Esteban, R. and Wickner, R.B. 1986, In vitro L-A double-stranded RNA synthesis in virus-like particles from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 83:4433–4437.

    Article  PubMed  CAS  Google Scholar 

  • Fujimura, T. and Wickner, R.B. 1988, Gene overlap results in a viral protein having an RNA binding domain and a major coat protein domain. Cell. 55:663–671.

    Article  PubMed  CAS  Google Scholar 

  • Gacto, M., Vicente Soler, J and Pardo, C. 1992, Production and characterization of pectic enzymes from yeasts, pp. 173–181 In: Villa, T.G. and Abalde, J. (eds.) Profiles on Biotechnology, Santiago University Press, Spain.

    Google Scholar 

  • Galzy, P. 1958, Origine de la microflore des vins. Proc. Agric. Vitic. 75:58–65.

    Google Scholar 

  • Gillian, M.H. and Fleet, G.H. 1985, Growth of natural flora during fermentation of inoculated wines. Appl. Env. Microbiol. 50:727–728.

    Google Scholar 

  • Girond, S., Blazy-Mougen, F. and Michell, G 1989, Influence de quelque pesticides viticoles sur les levures et la fermentation. Rev. Fr. Oenol. 119:14–22.

    Google Scholar 

  • Gonzalez-Candelas, L., Cortell, A. and Ramon D. 1995, Construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene. FEMS Microbiol. Let. 126:263–270.

    Article  CAS  Google Scholar 

  • Goto, S. 1980, Changes in the wild yeast flora of sulfited grape musts. J. Inst. Enol. Vitic. 15:29–32.

    Google Scholar 

  • Goto, S., Oguri, H. and Yamazaki, M. 1984, Yeast population in botrytised grapes. J. Inst. Enol. Vitic. 19:1–5.

    Google Scholar 

  • Guerzoni, E. and Marchetti, R. 1987, Analysis of the yeast flora associated with grape sour rot and the chemical disease markers. Appl. Env. Microbiol. 53:571–576.

    CAS  Google Scholar 

  • Hansen, E.C. 1903, Neue Untersuchungen uber den Kreislanf der Hefernanten in der natur. Zentr. Bakteriol. 10:1–8.

    Google Scholar 

  • Heard, G.M. and Fleet, G.H. 1987, Occurrence and growth of killer yeasts during wine fermentation. Appl. Environ. Microbiol. 53:2171–2174.

    PubMed  CAS  Google Scholar 

  • Icho, T. and Wickner, R.B. 1989, The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames J. Biol. Chem. 252:6716–6723.

    Google Scholar 

  • Kunkee, R.E. and Amerine, M.H. 1970, Yeast in winemaking, pp. 5–71 In: Rose, A.H. and Harrison, J.S. (eds.) The Yeasts, Academic Press, New York.

    Google Scholar 

  • Longo, E., Velazquez, J.B., Cansado, J., Calo, P. and Villa, T.G. 1990, Role of killer effect in fermentations conducted by mixed strains of Saccharomyces cerevisiae. FEMS Microbiol. Let. 71:331–336.

    Article  CAS  Google Scholar 

  • Longo, E., Cansado, J., Agrelo, D. and Villa, T.G. 1991, Effect of climatic conditions on yeast diversity in grape musts from northwest Spain. Am. J. Enol. Vitic. 42:141–144.

    Google Scholar 

  • Longo, E., Cansado, J., Sieiro, C., Calo, P., Velazquez, J.B. and Villa T.G. 1992, Influence of the curing of the killer phenotype in Saccharomyces cerevisiae wine strains on their fermentative behaviour World J. Microbiol. Biotechnol. 8:147–150.

    Article  CAS  Google Scholar 

  • McKay, A.M. 1990, Degradation of polygalacturonic acid by Saccharomyces cerevisiae, Let. Appl. Microbiol. 11:41–44.

    CAS  Google Scholar 

  • Marchetti, R., Guerzoni, E. and Gentile, M. 1984, Reserche sur l’étiologie d’une nouvelle maladie de la grappe:la pourriture acide. Vitis. 23:55–65

    CAS  Google Scholar 

  • Mrak, E.M. and McClung, S. 1940, Yeasts occurring on grapes and grape products in California. J. Bacteriol. 40:395–399.

    PubMed  CAS  Google Scholar 

  • Monteil, H., Blazy-Mangen, F. and Michel, G. 1986, Influence des pesticides sur la croissance des levures des raisins et des vins. Science des Aliments. 6:349–360.

    CAS  Google Scholar 

  • Naumova, G.I. and Naumov T.I. 1973, Comparative genetics of yeasts. XIII. Study of antagonistic interrelations in Saccharomyces cerevisiae. Genetika 9:85–90.

    Google Scholar 

  • Ouchi, K., Wickner, R.B., Tohe, A. and Akiyama H. 1979, Breeding of killer yeast for saké brewing by cytoduction J. Ferment. Technol. 57:483–487.

    CAS  Google Scholar 

  • Palfree, R.G.E. and Bussey, H. 1979, Yeast killer toxin: purification and characterization of the protein from S. cerevisiae. Eur. J. Biochem. 93:487–493.

    Article  PubMed  CAS  Google Scholar 

  • Pasteur, L. 1857, Memoires sur la fermentation alcoolique. CR Acad. Scs. Paris. XLV:1032–1036.

    Google Scholar 

  • Perez-Gonzalez, J.A., Gonzalez, R., Querol, A., Sendra, J. and Ramon, D. 1993, Construction of a recombinant wine yeast strain expressing β-(1,4)-endoglucanase and its use in microvinification processes Appl. Environ. Microbiol. 59:2801–2806.

    PubMed  CAS  Google Scholar 

  • Phaff, H.J., Miller, M.W. and Mrak, E.M. 1978, The Life of Yeasts. Harvard University Press. Cambridge. Mass. USA.

    Google Scholar 

  • Radler, F. and Schmitt, M. 1987, Killer toxins of yeasts: inhibitors of fermentation and their adsorption. J. Food. Protect. 50:234–238.

    CAS  Google Scholar 

  • Ribereau-Gayon, P., Peynaud, E., et Sudraud, P. 1978. Sciences et Techniques du Vin (vol. 2) Dunod. Paris.

    Google Scholar 

  • Ribereau-Gayon, P. 1985, New developments in wine microbiology. Am. J. Enol. Vitic. 36:1–10.

    CAS  Google Scholar 

  • Rossini, G., Federici, F. and Martini, A. 1982, Yeast flora of grape berries during ripening. Microb. Ecol. 8:83–89.

    Article  Google Scholar 

  • Rossini, G. 1986, Effect of killer strains on winemaking yeast starters. Ann. Fc. Agrar. Univ. Stud. Perugia. 38:345–354.

    Google Scholar 

  • Sapis-Domerq, S., Bertrand, A., Mur, F. and Sarre, C. 1977, Influence des produits de traitement de la vigne sur la microflore des raisins et des vins. Conn. Vigne Vin. 11:227–242.

    Google Scholar 

  • Seki, T., Choi, E. and Ryu, D. 1985, Construction of killer wine yeast strain. Appl. Environ. Microbiol. 49:1211–1215

    PubMed  CAS  Google Scholar 

  • Soumalainen, H. and Lehtonen, M. 1979, The production of aroma compounds by yeasts. J. Inst. Brewing 85:149–156.

    Google Scholar 

  • Starmer, W.T., Ganter, P.F., Aberdeen, V., Lachance, M.A. and Phaff, H.J. 1987, The ecological role of killer yeast in natural communities of yeasts. Can. J. Microbiol. 33:783–796.

    Article  PubMed  CAS  Google Scholar 

  • Sturley, S.L., Elliot, Q., Le Vietre, J., Tipper, D.J. and Bostian, K.A. 1986, Map** of functional domains within the S. cerevisiae type I killer preprotoxin. EMBO J. 5:3381–3389.

    PubMed  CAS  Google Scholar 

  • Suihko, M.L., Lehtinen, U., Zurbriggen, B., Kilpola, A., Nowles, J. and Penttla, M. 1991, Construction and analysis of recombinant glucanolytic brewer’s yeast strains. Appl. Microbiol. Biotechnol. 35:781–787.

    Article  CAS  Google Scholar 

  • Templeton, M.D., Sarrock, K.R., Bowen J.K., Crowhurst, R.N. and Rikkerink, E.H.A. 1994, The pectin lyase gene (pnl) family from Glomerella cingulata: characterization of pnlA and its expression in yeast. Gene 142:141–146.

    Article  PubMed  CAS  Google Scholar 

  • Tiourina, L.V., Bourjan, N.I. and Skarikova T.K. 1980, Emploi des cultures pures du phenotype killer dans la fermentation des mouts de raisin. Bull. O.I.V. 593:573–576.

    Google Scholar 

  • Tipper, D.J. and Schmitt, M.J. 1991, Yeast ds-RNA viruses replication and killer phenotypes. Mol. Microbiol. 5:2331–2338.

    Article  PubMed  CAS  Google Scholar 

  • van Zyl, J.A. and Du Plessis, L. 1961, The microbiology of South African winemaking. Part 1. The yeasts occuring in vineyards, musts and wines. S. Afr. J. Agric. Sci. (Pretoria) 4:393–401.

    Google Scholar 

  • Welsh, D.J. and Leibowitz, M.J. 1980, Transcription of killer virion double-stranded RNA in vitro. Nucleic Acid Res. 8:2365–2375.

    Article  PubMed  CAS  Google Scholar 

  • Wickner, R.B. 1992, Yeast RNA virologyer systems, pp. 263–269 In: Broach, J.R., Pringle, J.R. and Jones, E.W. (eds.) The Molecular Biology of the Yeast Saccharomyces, vol. 1, Cold Spring Harbor Laboratory, New York, USA.

    Google Scholar 

  • Young, T.W. 1980, The genetic manipulation of killer character into brewing yeast. J. Inst. Brew. 87:292–295.

    Google Scholar 

  • Zhu, H. and Bussey, H. 1991, Mutational analysis of the functional domains of yeast K1 killer toxin. Mol. Cell. Biol. 11:175–181.

    PubMed  CAS  Google Scholar 

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Villa, T.G., Longo, E. (1996). Microbiology of Grape Surfaces and its Importance for Wine Quality. In: Morris, C.E., Nicot, P.C., Nguyen-The, C. (eds) Aerial Plant Surface Microbiology. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-34164-4_13

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  • DOI: https://doi.org/10.1007/978-0-585-34164-4_13

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