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Hydrogel-Based Multi-enzymatic System for Biosynthesis

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Cell-free Production

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 186))

Abstract

Biosynthesis involving multi-enzymatic reactions is usually an efficient and economic method to produce plentiful important molecules. To increase the product yield in biosynthesis, the involved enzymes can be immobilized to carriers for enhancing enzyme stability, increasing synthesis efficiency and improving enzyme recyclability. Hydrogels with three-dimensional porous structures and versatile functional groups are promising carriers for enzyme immobilization. Herein, we review the recent advances of the hydrogel-based multi-enzymatic system for biosynthesis. First, we introduce the strategies of enzyme immobilization in hydrogel, including the pros and cons of the strategies. Then we overview the recent applications of the multi-enzymatic system for biosynthesis, including cell-free protein synthesis (CFPS) and non-protein synthesis, especially high value-added molecules. In the last section, we discuss the future perspective of the hydrogel-based multi-enzymatic system for biosynthesis.

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Acknowledgments

The authors thank the financial support from Shenzhen Bay Laboratory (No. 21280031 and S211101001-1).

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  1. Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, Guangdong, China

    Han Wu & Bo Zheng

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  1. Han Wu
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Correspondence to Bo Zheng .

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Editors and Affiliations

  1. Department of Chemical Engineering, Tsinghua University, Bei**g, China

    Yuan Lu

  2. Department of Bioengineering, Stanford University, Stanford, CA, USA

    Michael C. Jewett

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Wu, H., Zheng, B. (2023). Hydrogel-Based Multi-enzymatic System for Biosynthesis. In: Lu, Y., Jewett, M.C. (eds) Cell-free Production. Advances in Biochemical Engineering/Biotechnology, vol 186. Springer, Cham. https://doi.org/10.1007/10_2023_220

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