Abstract
In this report, two microfabricated chip electrophoresis techniques and several application studies were tested for rapid and high-resolution separation of double-stranded (ds)DNA. In one technique, low-viscosity hydroxypropylmethylcellulose-50 (HPMC-50) matrix accompanied by polyhydroxy compounds, such as mannitol, glucose, and glycerol, showed higher resolving power than conventionally and singly used HPMC-50 matrix. The new matrix is easy to be hyphenated in the future μ-TAS platforms. Another technique is through the stepwise (multi-step) filling of different concentrations of one polymer or different types of polymers to achieve high-resolution separation of both short and long DNA fragments simultaneously. The technique has good migration-time reproducibility for the analysis of restriction digest fragments and ladders. The separation application of some PCR products was performed on an Agilent 2100 Bioanalyzer. The reproducibility and accuracy of fragment sizing of a DNA ladder were satisfactory. Fast analysis of DNA polymorphisms on the human Y-chromosome was realized with the analytical time of three genomic polymorphisms on the Y-chromosome (Y Alu polymorphism, 47z/StuI and 12 f2) to be within 110 s, respectively. A mixture of nine DNA markers on the human Y-chromosome related to examine the cause of spermatogenic failure was successfully separated with the smallest fragment size difference of 7 bp.
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Xu, F., Zhang, L., Jabasini, M., Baba, Y. (2006). Microfabricated Chip Electrophoresis Technology for DNA Analysis. In: **ng, WL., Cheng, J. (eds) Frontiers in Biochip Technology. Springer, Boston, MA. https://doi.org/10.1007/0-387-25585-0_6
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DOI: https://doi.org/10.1007/0-387-25585-0_6
Publisher Name: Springer, Boston, MA
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