Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1008)
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About this book
Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.
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Keywords
Table of contents (19 protocols)
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Introduction and Overview
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Quantitation of Thermodynamics and Kinetics
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Spectroscopic Methods
Editors and Affiliations
Bibliographic Information
Book Title: Protein-Ligand Interactions
Book Subtitle: Methods and Applications
Editors: Mark A. Williams, Tina Daviter
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-398-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-397-8Published: 01 June 2013
Softcover ISBN: 978-1-4939-5873-3Published: 17 November 2016
eBook ISBN: 978-1-62703-398-5Published: 01 June 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: X, 530
Number of Illustrations: 101 b/w illustrations, 45 illustrations in colour