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Haemostasis

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Paul Monagle

    1. Department of Paediatrics, University of Melbourne, Parkville, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 992)

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About this book

The haemostatic system is one the most important physiological systems for maintaining health and well being, and thus the investigation of the haemostatic system remains a research priority. Disturbances of the haemostatic system in the broader sense, such as heart disease and strokes, arguably constitute the single greatest contribution to non-infectious mortality in the world today. Therefore, understanding the laboratory methods to assess the haemostatic system is vital for the practice of complex clinical medicine. In Haemostasis: Methods and Protocols, experts in the field address the major components of the haemostatic system, general principles of haemostatic testing, and techniques used to assess various aspects of the haemostatic system, grouped according to their functional indications. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Haemostasis: Methods and Protocols provides an ideal guide to scientists of all backgrounds and serves an urgent need for further research to develop superior methods of assessing the haemostatic system in humans.

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xii
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  2. The Haemostatic System

    1. Front Matter

      Pages 1-1
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    2. The Coagulation System in Humans

      • Anthony K. C. Chan, Nethnapha Paredes
      Pages 3-12

    3. Platelet Physiology

      • Matthew D. Linden
      Pages 13-30

    4. The Role of the Vessel Wall

      • Nethnapha Paredes, Anthony K. C. Chan
      Pages 31-46

  3. General Principles of Haemostatic Testing

    1. Front Matter

      Pages 47-47
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    2. Specimen Requirements for the Haemostasis Laboratory

      • Linda J. Stang, Lesley G. Mitchell
      Pages 49-71

    3. Methods of Coagulation

      • Gemma Crighton
      Pages 73-83

    4. Reference Ranges of Coagulation Tests

      • Oliver Speer, Markus Schmugge, Claudia Metzger, Manuela Albisetti
      Pages 85-96

    5. Lupus Anticoagulant Testing

      • Karen Moffat, Anne Raby, Mark Crowther
      Pages 97-108

  4. Examples of Global Tests of Haemostasis

    1. Front Matter

      Pages 109-109
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    2. Activated Partial Thromboplastin Time

      • Vera Ignjatovic
      Pages 111-120

    3. Prothrombin Time/International Normalized Ratio

      • Vera Ignjatovic
      Pages 121-129

    4. Thrombin Clotting Time

      • Vera Ignjatovic
      Pages 131-138

    5. Thrombin Generation

      • Leslie R. Berry, Anthony K. C. Chan
      Pages 139-154

    6. Activated Clotting Time (ACT)

      • Stephen Horton, Simon Augustin
      Pages 155-167

  5. Examples of Tests for Bleeding Disorders

    1. Front Matter

      Pages 169-169
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    2. Factor XIII Assays

      • Stephen Opat, Jenny Butler, Erica Malan, Elizabeth Duncan, Huyen A. M. Tran
      Pages 171-180

    3. Fibrinogen

      • Linda J. Stang, Lesley G. Mitchell
      Pages 181-192

    4. Platelet Counting

      • Sylvain Salignac, Véronique Latger-Cannard, Nicole Schlegel, Thomas Pierre Lecompte
      Pages 193-205
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Editors and Affiliations

  • Department of Paediatrics, University of Melbourne, Parkville, Australia

    Paul Monagle

Bibliographic Information

  • Book Title: Haemostasis

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Monagle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-339-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-338-1Published: 02 April 2013

  • Softcover ISBN: 978-1-4939-6102-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-339-8Published: 02 April 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 431

  • Topics: Molecular Medicine, Hematology

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