Overview
- Editors:
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Karen Mossman
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Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada
- Includes cutting-edge methods and protocols for the study of innate antiviral immunity
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
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About this book
This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Â
Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.
The chapter 'Morphological Separation ofClustered Nuclei in Histological Images' is published open access under a CC BY 4.0 license at springer.longhoe.net.Â
Table of contents (19 protocols)
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- Fatemeh Vahedi, Elizabeth C. Giles, Ali A. Ashkar
Pages 1-56
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- Chandra S. Mukhopadhyay, Ramneek Verma, Jasdeep Singh
Pages 89-102
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- Sarah J. Poynter, Stephanie J. DeWitte-Orr
Pages 103-118
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- Renee C. Ireton, Courtney Wilkins, Michael Gale Jr.
Pages 119-129
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- Christine Vazquez, Dia C. Beachboard, Stacy M. Horner
Pages 131-142
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- Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel
Pages 143-152Open Access
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- Jennifer Paijo, Volkhard Kaever, Ulrich Kalinke
Pages 153-166
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- Vladislav Pokatayev, Nan Yan
Pages 167-174
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- Shiyou Zhu, Yuexin Zhou, Wensheng Wei
Pages 175-181
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- Bryan J. Gall, Victor R. DeFilippis
Pages 183-193
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- Yoon Jung Kim, Tae Hoon Kim
Pages 195-208
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- Gabriel A. Leiva-Torres, Nestor Nebesio, Silvia M. Vidal
Pages 209-227
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- Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib
Pages 229-251
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- Claudia U. Duerr, Jörg H. Fritz
Pages 253-261
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- Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel
Pages E1-E1
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- Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib
Pages E3-E3
Editors and Affiliations
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Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada
Karen Mossman