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C. elegans

Methods and Applications

  • Book
  • © 2015
  • Latest edition

Overview

Editors:
  1. David Biron

    1. Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Gal Haspel

    1. Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1327)

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About this book

The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Library Construction for Mutation Identification by Whole-Genome Sequencing

    • Harold E. Smith
    Pages 1-9

  3. Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes

    • Eric S. Haag, Cristel G. Thomas
    Pages 11-21

  4. Genetic Methods for Cellular Manipulations in C. elegans

    • Menachem Katz
    Pages 23-38

  5. A Fusion PCR Method for Expressing Genetic Tools in C. elegans

    • Yifat Eliezer, Alon Zaslaver
    Pages 39-47

  6. Transposon-Assisted Genetic Engineering with Mos1-Mediated Single-Copy Insertion (MosSCI)

    • Christian Frøkjær-Jensen
    Pages 49-58

  7. Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System

    • John A. Calarco, Ari E. Friedland
    Pages 59-74

  8. Observing and Quantifying Fluorescent Reporters

    • Michael Hendricks
    Pages 75-85

  9. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior

    • Caspar Glock, Jatin Nagpal, Alexander Gottschalk
    Pages 87-103

  10. Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans

    • Nicholas F. Trojanowski, Christopher Fang-Yen
    Pages 105-119

  11. High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans

    • Laura Manning, Janet Richmond
    Pages 121-140

  12. Electron Tomography Methods for C. elegans

    • David H. Hall, William J. Rice
    Pages 141-158

  13. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans

    • Ross C. Lagoy, Dirk R. Albrecht
    Pages 159-179

  14. Tracking Single C. elegans Using a USB Microscope on a Motorized Stage

    • Eviatar I. Yemini, André E. X. Brown
    Pages 181-197

  15. An Imaging System for C. elegans Behavior

    • Matthew A. Churgin, Christopher Fang-Yen
    Pages 199-207

  16. A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae

    • Maria C. Ow, Sarah E. Hall
    Pages 209-219

  17. Sampling and Isolation of C. elegans from the Natural Habitat

    • Nausicaa Poullet, Christian Braendle
    Pages 221-229

  18. A Primer on Prototy**

    • Dylan Lynch, David Biron
    Pages 231-240

  19. A Primer on Quantitative Modeling

    • Iulia Neagu, Erel Levine
    Pages 241-250

  20. Back Matter

    Pages 251-252
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Editors and Affiliations

  • Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA

    David Biron

  • Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, USA

    Gal Haspel

Bibliographic Information

  • Book Title: C. elegans

  • Book Subtitle: Methods and Applications

  • Editors: David Biron, Gal Haspel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2842-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2841-5Published: 02 October 2015

  • Softcover ISBN: 978-1-4939-4892-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2842-2Published: 30 September 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 252

  • Number of Illustrations: 13 b/w illustrations, 44 illustrations in colour

  • Topics: Human Genetics, Animal Genetics and Genomics, Animal Models

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