Abstract
Satsuma mandarin (Citrus unshiu Marc.) is characterized by its tender peel and seedless nature. The representative cultivar of Satsuma mandarin is Miyagawa wase. Another cultivar, Ueno wase was developed from the bud mutation of a Miyagawa wase tree. Compared to Miyagawa wase fruit, Ueno wase fruit is known to have less albedo, higher sugar content, and to mature earlier. To find genes related to this bud mutation, a citrus 300 k DNA microarray was developed for gene expression analyses of the fruits and leaves of each cultivar. When up-regulated genes were classified in the Cluster of Orthologous Groups of proteins database, the number of genes included differed significantly between the two cultivars. Ten genes, including cytochrome P450, α-mannosidase, S-adenosylmethionine decarboxylase, carboxyesterase, anthranilate synthase, glycosyl hydrolase family-17, ethylene response factor subfamily, and alcohol dehydrogenase, showing significant differences in gene expression by DNA microarray were selected, and their expression patterns were examined by RT-PCR. The information gained from genes that are up (or down)-regulated upon bud mutation may explain the observed phenotypes, while also increasing the understanding of genes related to citrus bud mutation.
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References
Abbott A, Georgi L, Yvergniaux D, Wang Y, Blenda A, Reighard G, Inigo M, and Sosinski B(2002) Peach the model genome for Rosaceae. Acta Hortic 575, 145–155.
Andrews DL, MacAlpine DM, Johnson JR, Kelley PM, Cobb BG, and Drew MC (1994) Differential induction of mRNAs for the glycolytic and ethanolic fermentative pathways by hypoxia and anoxia in maize seedlings. Plant Physiol 106, 1575–1582.
Bowles DJ (1990) Defence-related proteins in higher plants. Ann Rev Biochem 59, 873–907.
Bruxelles GL, Peacock de WJ, Dennis ES, and Dolferus R, (1996) Abscisic acid induces the alcohol dehydrogenase gene in Arabidopsis. Plant Physiol 111, 381–391.
Cummins I, Landrum M, Steel PG, and Edwards R (2007) Structure activity studies with xenobiotic substrates using carboxylesterases isolated from Arabidopsis thaliana. Phytochem 68, 811–818.
Cuzin M (2001) DNA chips: a new tool for genetic analysis and diagnostics. Transfus Clin et Biol 8, 291–296.
Dubouzet JG, Ishihara A, Matsuda F, Miyagawa H, Iwata H, and Wakasa K (2007) Integrated metabolomic and transcriptomic analyses of high-tryptophan rice expressing a mutant anthranilate synthase alpha subunit. J Exp Bot 58, 3309–3321.
Duan H and Schuler MA (2005) Differential expression and evolution of the Arabidopsis CYP86A subfamily. Plant Physiol 137, 1067–1081.
Duggan DJ, Bittner M, Chen Y, Meltzer P, and Trent JM (1999) Expression profiling using cDNA microarrays. Nat Genet 21, 10–14.
Emmanuel E and Levy AA (2002) Tomato mutants as tools for functional genomics. Curr Opin Plant Biol 5, 112–117.
Federici C, Fang DQ, Scora RW, and Roose ML (1998) Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theor Appl Genet 96, 812–822.
Gandia M, Conesa A, Ancillo G, Gadea J, Forment J, Pallas V, Flores R, Duran-Vila N, Moreno P, and Guerri J (2008) Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus. Virol 25, 298–306.
Gershater MC, Cummins I, and Edwards R (2007) Role of a carboxylesterase in herbicide bioactivation in Arabidopsis thaliana. J Bio Chem 282, 21460–21466.
Girke T, Todd J, Ruuska S, White J, Benning CH, and Ohlrogge J (2000) Microarray analysis of develo** Arabidopsis seed. Plant Physiol 124, 1570–1581.
Grosser JW and Gmitter Jr FG (1990) Protoplast fusion and citrus improvement. Plant Breed Rev 8, 339–374.
Hiroshi F, Takehiko S, Aiko S, Fumie N, Tomoko E, Michiharu N, Yoshinori I, Tokurou S, and Mitsuo O (2007) Profiling ethylene-responsive genes in mature mandarin fruit using a citrus 22K oligoarray. Plant Sci 173, 340–348.
Irizarry RA, Bolstad B, Collin F, Cope L, Hobbs M, and Speed T (2003) Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Res 15, e15
James S (1990) In Citrus Varieties of The World, pp. 35–38. Sinclair International Limited. Norwich, UK.
Jenks MA, Joly RJ, Peters PJ, Rich PJ, Axtell JD, and Ashworth E. N (1994) Chemically induced cuticle mutation affecting epidermal conductance to water vapor and disease susceptibility in Sorghum bicolor (L.) Moench. Plant Physiol 105, 1239–1245.
Kauffmann S, Legrand M, Geoffroy P, and Fritig B (1987) Biological function of ’pathogenesis-related’ proteins: Four PR proteins of tobacco have 1, 3-β-glucanase activity. EMBO J 6, 3209–3213.
Kijas J, Thomas M, Fowler J, and Roose M (1997) Integration of trinucleotide microsatellites into a linkage map of Citrus. Theor Appl Genet 94, 701–706.
Kim YJ and Hwang BK (1997) Isolation of a basic 34 kilodalton β-1,3-glucanase with inhibitory activity against Phytophthora capsici from pepper stems. Physiol Mol Plant Pathol 50, 103–115.
Lawrence CJ, Dong Q, Polacco ML, Seigfred TE, Brendel V, and Maize GDB (2004) The community database for maize genetics and genomics. Nucleic Acids Res 32 (Database issue), 393–397.
Lee YP, Yu GH, Seo YS, Han SE, Choi YO, Kim D, Mok IG, Kim WT, and Sung SK (2007) Microarray analysis of apple gene expression engaged in early fruit development. Plant Cell Rep 26, 917–926.
Manríquez D, El-Sharkawy I, Flores FB, El-Yahyaoui F, Regad F, Bouzayen M, Latché A, and Pech JC (2006) Two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics. Plant Mol Biol 61, 675–685.
Mauch F, Mauchmani B, and Boller T (1988) Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiol 88, 936–942.
McCollum TG and McDonald EE (1991) Electrolyte leakage, respiration, and ethylene production as indices of chilling injury in grapefruit. Hort Sci 26, 1191–1192.
Nakamura K, Inoue M, Yoshiie T, Hosoi K, and Kimura Y (2008) Changes in structural features of free N-glycan and endoglycosidase activity during tomato fruit ripening. Bioscience Biotechnology and Biochem 72, 2936–2945.
Nakano T, Suzuki K, Fujimura T, and Shinshi H (2006) Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol 140, 411–432.
Ohlrogge J and Browse J (1995) Lipid biosynthesis. Plant Cell 7, 957–970.
Pastuszak I, Kaushal GP, Wall KA, Pan YT, Sturm A, and Elbein AD (1990) Purification and properties of arylmannosidases from mung bean seedlings and soybean cells. Glycobiol 1, 71–82.
Piffanelli P, Ross JH, and Murphy DJ (1997) Intra- and extracellular lipid composition and associated gene expression patterns during pollen development in Brassica napus. Plant J 11, 549–562.
Riechmann JL, Heard J, Martin G, Reuber L, Jiang CZ, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman B, and Yu GL (2000) Arabidopsis Transcription Factors: Genome-Wide Comparative Analysis Among Eukaryotes. Science 15, 2105–2110.
Rudy D, Gérard M, and Michel J (1985) Alcohol dehydrogenase in Arabidopsis: analysis of the induction phenomenon in plants and tissue cultures. Mol Gen Genet 199, 256–264.
Ruiz C and Asins MJ (2003) Comparison between Poncirus and Citrus genetic linkage maps. Theor Appl Genet 106, 826–836.
Schaffer R, Landgraf J, Accerbi M, Simon VV, Larson M, and Wisman E (2001) Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis. Plant Cell 13, 113–123.
Schena M, Shalon D, Heller R, Chai A, Brown P, and Davis RW (1996) Parallel human genome analysis: microarray based expression monitoring of 1000 genes. Proc Natl Acad Sci USA 93, 10614–10619.
Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, and Shinozaki K (2001) Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell 13, 61–72.
Speirs J, Lee E, Holt K, Yong-Duk K, Scott N, Loveys B, and Schuch W (1998) Genetic manipulation of alcohol dehydrogenase levels in ripening tomato fruit affects the balance of some flavor aldehydes and alcohols. Plant Physiol 117, 1047–1058.
Takehiko S, Fuiii H, Endo T, Yazaki J, Kishimoto N, Shimbo K, Kikuchi S, and Omura M (2005) Toward comprehensive expression profiling by microarray analysis in citrus: monitoring the expression profiles of 2213 genes during fruit development. Plant Sci 168, 1383–1385.
Walden R, Cordeiro A, and Tiburcio AF (1997) Polyamines: small molecules triggering pathways in plant growth and development. Plant Physiol 113, 1009–1013.
Wellesen K, Durst F, Pinot F, Benveniste I, Nettesheim K, Wisman E, Steiner-Lange S, Saedler H, and Yephremov A (2001) Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid x-hydroxylation in development. Proc Natl Acad Sci USA 98, 9694–9699.
Whetten R, Sun YH, Zhang Y, and Sederoff R (2001) Functional genomics and cell wall biosynthesis in loblolly pine. Plant Mol Biol 47, 275–291.
Woo KK and Kimura Y (2005) Regulation of substrate specificity of plant α-mannosidase by cobalt ion: in vitro hydrolysis of high-mannose type N-glycans by Co2+-activated Ginkgo α-mannosidase. Biosci Biotechnol Biochem 69, 1111–1119.
Workman C, Jensen LJ, Jarmer H, Berka R, Gautier L, Nielser HB, Saxild HH, Nielsen C, Brunak S, and Knudsen S (2002) A new non-linear normalization method for reducing variability in DNA microarray experiments. Genome Biol 30, 48–56
Yamaguchi T, Nakayama K, Hayashi T, Tanaka Y, and Koike S (2002) Molecular cloning and characterization of a novel â-1, 3-glucanase gene from rice. Biosci Biotechnol Biochem 66, 1403–1406.
Yamakawa H, Kamada H, Satoh M, and Ohashi Y (1998) Spermine is a salicylate-independent endogenous inducer for both tobacco acidic pathogenesis-related proteins and resistance against tobacco mosaic virus infection. Plant Physiol 118, 1213–1222.
Yang YH (1994) In Citrus Fruits Illustrated of Cheju, pp. 27–51. Dae-Young Press, Seoul, Korea.
Yang SF and Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35, 155–189.
Yazaki J, Shimatani Z, Hashimoto A, Nagata Y, Fujii F, Kojima K, Suzuki K, Taya T, Tonouchi M, Nelson C, Nakagawa A, Otomo Y, Murakami K, Matsubara K, Kawai J, Carninci P, Hayashizaki Y, and Kikuchi S (2004) Transcriptional profiling of genes responsive to abscisic acid and gibberellin in rice: phenoty** and comparative analysis between rice and Arabidopsis. Physiol Genomics 17, 87–100.
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Jeong-Won Park and Kyung-Hwan Boo contributed equally to
Jeong-Won Park and Kyung-Hwan Boo contributed equally to
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Park, JW., Boo, KH., **, SB. et al. Expression profiling of cultivar-related genes in Satsuma mandarins, Miyagawa wase and Ueno wase. J. Korean Soc. Appl. Biol. Chem. 53, 691–701 (2010). https://doi.org/10.3839/jksabc.2010.105
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DOI: https://doi.org/10.3839/jksabc.2010.105