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Determination of indole-3-carbinol and indole-3-acetonitrile in Brassica vegetables using high-performance liquid chromatography with fluorescence detection

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Abstract

A simple and sensitive method for determining indole-3-carbinol (I3C) and indole-3-acetonitrile (I3A) from Brassica vegetable was developed using high-performance liquid chromatography with fluorescence detection. The indolic glucosinolate as well as I3C and I3A concentrations were found to be significantly higher in Brassica rapa L. ssp. pekinensis than in B. Juncea var. integrifolia.

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References

  • Anderton MJ, Jukes R, Lamb JH, Manson MM, Gescher A, Steward WP, and Williams ML (2003) Liquid chromatographic assay for the simultaneous determination of indole-3-carbinol and its acid condensation products in plasma. J Chromatogr B 787, 281–291.

    Article  CAS  Google Scholar 

  • Bradfield CA and Bjeldanes LF (1987) High-performance liquid chromatographic analysis of anticarcinogenic indoles in Brassica oleracea. J Agr Food Chem 35, 46–49.

    Article  CAS  Google Scholar 

  • Calatayud JM, De Ascençõ JG, and Albert G JR (2006) FIA-fluorimetric determination of the pesticide 3-indolyl acetic acid. J Fluoresc 16, 61–67.

    Article  CAS  Google Scholar 

  • De Vos MK, Ksenia L, and Jander G (2008) Indole-3-acetonitrile production from indole glucosinolates deters oviposition by Pieris rapae. Plant Physiol 146, 916–926.

    Article  Google Scholar 

  • European Community (1990) Oil seeds-determination of glucosinolates by high performance liquid chromatography. Official J Europ Comm L170, 27–34.

    Google Scholar 

  • Fahey JW, Zalcmann AT, and Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56, 5–51.

    Article  CAS  Google Scholar 

  • Jongen WMF (1996) Glucosinolates in Brassica: occurrence and significance as cancer-modulating agents. Proc Nutr Soc 55, 433–446.

    Article  CAS  Google Scholar 

  • Kim JK, Chu SM, Kim SJ, Lee DJ, Lee SY, Lim SH, Ha S-H, Kweon SJ, and Cho HS (2010) Variation of glucosinolates in vegetable crops of Brassica rapa L. ssp. pekinensis. Food Chem 119, 423–428.

    Article  CAS  Google Scholar 

  • Maiyoh GK, Kuh JE, Casaschi A, and Theriault AG (2007) Cruciferous indole-3-carbinol inhibits apolipoprotein B secretion in HepG2 cells. J Nutr 137, 2185–2189.

    CAS  Google Scholar 

  • Pedras MSC, Nycholat CM, Montaut S, Xu Y, and Khan AQ (2002) Chemical defenses of crucifers: elicitation and metabolism of phytoalexins and indole-3-acetonitrile in brown mustard and turnip. Phytochemistry 59, 611–625.

    Article  CAS  Google Scholar 

  • Shim KH, Kang KS, Sung NK, Seo KI, and Moon JS (1992) Formation of indole glucosinolates breakdown products during processing treatment in cruciferous vegetables. J Korean Soc Food Nutr 21, 49–53.

    CAS  Google Scholar 

  • Slominski BA and Campbell LD (1987) Gas chromatographic determination of indole glucosinolates -A re-examination. J Sci Food Agr 40, 131–143.

    Article  CAS  Google Scholar 

  • Tolonen M, Taipale M, Viander B, Pihlava JM, Korhonen H, and Ryhänen EL (2002) Plant-derived biomolecules in fermented cabbage. J Agr Food Chem 50, 6798–6803.

    Article  CAS  Google Scholar 

  • Zywicki B, Catchpole G, Draper J, and Fiehn O (2005) Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoes. Anal Biochem 336, 78–186.

    Article  Google Scholar 

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Correspondence to Jae Kwang Kim.

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Lee, S.Y., Chu, S.M., Lee, S.M. et al. Determination of indole-3-carbinol and indole-3-acetonitrile in Brassica vegetables using high-performance liquid chromatography with fluorescence detection. J. Korean Soc. Appl. Biol. Chem. 53, 249–252 (2010). https://doi.org/10.3839/jksabc.2010.039

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  • DOI: https://doi.org/10.3839/jksabc.2010.039

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