Conclusions
In summary, a free-standing MXene/CTS/Cu2O electrode was formed through electrostatic interaction of MXene and CTS with opposite charges, followed by the electrodeposition of Cu2O. Taking advantage of the synergistic function of MXene/CTS layers and Cu2O nanoparticles, this ternary electrode exhibits excellent sensing capabilities for glucose and cholesterol with preferable linear ranges that can cover the full concentration range in clinical diagnosis. For glucose sensing, the sensitivity was 60.295 µA·L/(mmol·cm2) with LOD being 52.4 µmol/L (SNR=3), while a sensitivity up to 215.71 µA·L/(mmol·cm2) and LOD low to 49.8 µmol/L (SNR=3) were achieved for cholesterol detection. Additionally, this biosensor possesses superior anti-interference ability and reproductivity, and thus exhibits great potential for genuine sample analysis. Accordingly, the as-prepared enzyme-free MXene/CTS/Cu2O electrode acts as a biomimetic electrocatalyst with excellent performance for analysis of multiple metabolites, and overcomes the disadvantages of an enzyme-based biosensor. This work has proposed a versatile strategy for designing and fabricating selfassembled nanocomposite materials with tuned structural and functional properties. It is a first attempt which could be easily integrated into portable electrochemical devices, facilitating effective routine monitoring of blood metabolites and paving the way for commercialization and point-of-care testing.
概要
目的
研制低成本、高精度的多种代谢物同时检测的生物传感器对医疗诊断具有重要意义。在本工作中,我们提出了一种基于MXene/壳聚糖(CTS)/Cu2O纳米复合材料的独立无酶电极,用于同时高精度测定葡萄糖和胆固醇。
创新点
1. 利用MXene、CTS和Cu2O的协同作用,通过电位分离,实现对葡萄糖和胆固醇的无酶同时检测;2. 优化检测范围,可用于检测人体血液样本。
方法
1. 通过MXene、CTS和Cu2O纳米材料的协同作用形成有效的界面结,促进反应过程中的电荷转移,进而提高与待测物质的接触面积;2. 分析电化学反应过程,构建电流信号与待测物浓度之间的关系,得到传感器的性能参数。
结论
1. MXene/CTS薄膜具有较高的比表面积,为离子的扩散提供了更多的通道,同时Cu2O纳米粒子可以提供丰富的金属活性边缘,促进了电荷转移,提高了反应活性。2. Cu2O纳米颗粒在胆固醇检测中发挥了重要作用,而同时作为导电基底和葡萄糖氧化剂的MXene,通过协同作用实现了在不同电位上同时无酶检测葡萄糖和胆固醇。3. 在优化的电位范围内(−0.80–0.40 V),该传感器对葡萄糖和胆固醇具有良好的线性响应,灵敏度分别为60.295和215.71 µA∙L/(mmol·cm2),而检出限分别为52.4和49.8 µmol/L。4. 通过对人血清样品的实时分析,验证了其良好的抗干扰能力和回收率(98.04%–102.94%),所以该传感器具有一定的临床应用前景。
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Acknowledgments
This work is supported by the National Key Scientific Instrument and Equipment Development Project of China (No. 51627808), the National Natural Science Foundation of China (No. 51605088), the Natural Science Foundation of Jiangsu Province (Nos. BK20170667 and BK20201278), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (No. SJCX20_0026), and the Zhishan Youth Scholar Program of Southeast University (SEU), China.
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Tao HU designed the research. Man ZHANG processed the corresponding data. Hui DONG and Tong LI participated in the methodology and discussion. **ao-bei ZANG provided the original technique of experiments and reviewing. Man ZHANG wrote the first draft of the manuscript. Zhong-hua NI and **ao LI helped to organize the manuscript. Man ZHANG and **ao LI revised and edited the final version.
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Tao HU, Man ZHANG, Hui DONG, Tong LI, **ao-bei ZANG, **ao LI, and Zhong-hua NI declare that they have no conflict of interest.
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All procedures were in accordance with the ethical standards of the Responsible Committee on Human Experimentation (Institute of Process Engineering, Chinese Academy of Sciences, China) and with the Helsinki Declaration of 1975, as revised in 2008(5). Informed consent was obtained from all patients for being included in the study.
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Free-standing MXene/chitosan/Cu2O electrode: an enzyme-free and efficient biosensor for simultaneous determination of glucose and cholesterol
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Hu, T., Zhang, M., Dong, H. et al. Free-standing MXene/chitosan/Cu2O electrode: an enzyme-free and efficient biosensor for simultaneous determination of glucose and cholesterol. J. Zhejiang Univ. Sci. A 23, 579–586 (2022). https://doi.org/10.1631/jzus.A2100584
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DOI: https://doi.org/10.1631/jzus.A2100584