Abstract
This study effectively utilizes ion-sensitive field-effect transistor (ISFET) technology to detect enzymatic oxidation reactions. The research presents a method for immobilizing glucose oxidase (GOD) enzymes by activating groups that covalently attach to magnetite nanoparticles coated with a silicon oxide containing carboxyl groups (F-NPs). Amperometry techniques are utilized to detect glucose concentrations on the ISFET gate area through incubation with carbodiimides (EDC) and succinimides (NHS). The ISFET device comprises the organic semiconductors (P3HT/PC71BM) on an indium tin oxide (ITO) coated polyethylene terephthalate (PET) substrate for source and drain electrodes. A PET-ITO gate electrode improves the gate configuration by utilizing dual gates: one on the semiconductor layer and another on a laser-induced graphene (LIG) pattern, interconnected for immobilization platform (PI) which can detect changes in solution load different glucose levels. IV saturation curves display a continual ISFET drain current decrease with increasing glucose concentration, confirming efficacy as a glucose sensor.
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Data availability
Experiments and measurements were carried out in two teaching laboratories: ESFM and CNMN, which are both affiliated with the Instituto Politecnico Nacional. The following personnel conducted the measurements: M. Sc. Luis Alberto Moreno Ruiz carried out the FTIR spectroscopy, the TEM and SEM analysis were performed Ph.D Hugo Martínez Gutierrez and Ph.D Nicolas Cayetano Castro. For access to the data produced or examined in this study, please consult the responsible persons upon reasonable request. Additionally, for comprehensive details about the processes of synthesis, characterization, and device construction, please refer to the postgraduate thesis repository. The study will be entitled “Funcionalización de nanopartículas magneticas para la inmobilización de antígenos” and will be part of the PhD program in Nanosciences and Micro-Nanotechnologies at the ENCB-IPN, with expected availability in June 2024.
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Acknowledgments
GLAB is grateful to Consejo Nacional de Humanidades Ciencias y Tecnologías (CONAHCyT) for its financial support throughout her adscription to the doctoral program of nanosciences. HMG and RGA are grateful to COFAA-IPN, EDD-IPN, and EDI-IPN for support through academic fellowships.
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The Instituto Politécnico Nacional (IPN) and the Secretaría de Investigación and Posgrado (SIP) supplied financial support for this research through projects numbered 20231885 and 20231318.
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All authors contributed to the study conception and design. GLAB performed the synthesis, device construction and the electrical and morphological analysis. JPAG and RGA collected electrical data for the IV curves. HMG acquired micrographs of the materials. The first draft of the manuscript was written by GLAB, and all authors commented on earlier versions of the manuscript. All authors read and approved the final manuscript.
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Araujo-Bernal, G.L., Aguilar-González, J.P., Martínez-Gutiérrez, H. et al. Use of laser-induced graphene and magnetite nanoparticles as anchors in electrochemical glucose detection devices. MRS Advances 9, 161–167 (2024). https://doi.org/10.1557/s43580-024-00832-1
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DOI: https://doi.org/10.1557/s43580-024-00832-1