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Poloxamer 407/chitosan micelles can improve α-Tocopherol effect on oral keratinocytes proliferation

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Abstract

This study aimed to produce a Poloxamer 407/Chitosan polymeric system for the controlled release of α-TOC and evaluate its biological properties using normal oral keratinocytes. The production of the polymeric controlled release system involved loading of α-TOC and micellization of the isotropic P407 phase with subsequent encapsulation with chitosan. The chitosan coverage of the micelles was confirmed by the positive zeta potential (+ 12.8 mV). The micelles (TOC-P407-CH) developed had average particle sizes of 193.8 ± 51.0 nm (PDI 0.502 ± 0.068) (α-TOC 50 mg/mL—TOC50-P407-CH) and 714.5 ± 110.18 nm (PDI 0.886 ± 0.196) (α-TOC 200 mg/mL—TOC200-P407-CH) (P = 0.0018) with irregular morphology. The release test by UV–Vis demonstrated a slow and controlled release for TOC50-P407-CH (initial release peak of 82% after the first 5 h). The α-TOC-loaded micelles did not show any cytotoxicity in vitro (P > 0.05). Also, they promoted superior cell proliferation in NOE cells (P < 0.001). This study shows that a controlled release system developed with 50 mg/mL α-TOC has the potential to be used in epithelial cell regeneration.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgments

We gratefully acknowledge the support from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPQ (Inct Teranostica).

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Authors and Affiliations

  1. Dentistry Graduation Program, Universidade Federal da Paraíba, Paraiba, Brazil

    Ésio de C. Coelho Junior, Panmella P. Maciel, Isis de A. F. Muniz, Simone A. de Sousa, Ana M. G. Valença, André U. D. Batista, Jefferson M. de Lima, Lúcio R. C. Castellano & Paulo R. F. Bonan

  2. School of Dentistry, Centro Universitário de Ensino Superior da Paraíba, Paraiba, Brazil

    Hugo Y. R. Silva

  3. Laboratory of Biomaterials, Dentistry Graduation Program, Universidade Federal da Paraíba, Paraiba, Brazil

    Rebeca T. A. Dias & André U. D. Batista

  4. Laboratory of Materials and Biosystems, Material Engineering Graduation Program, Universidade Federal da Paraíba, Paraiba, Brazil

    Lucas R. F. Figueiredo & Eliton S. de Medeiros

  5. Dentistry Graduation Program, Universidade Federal de Pernambuco, Permambuco, Brazil

    Jefferson M. de Lima & Danyel E. C. Perez

  6. Department of Otolaryngology - Head and Neck Surgery, McGill University, Montreal, Canada

    Jefferson M. de Lima & Sabrina D. da Silva

  7. Human Immunology Research and Education Group-GEPIH, Escola Técnica de Saúde da UFPB, Universidade Federal da Paraíba, Paraiba, Brazil

    Jefferson M. de Lima, Lúcio R. C. Castellano & Paulo R. F. Bonan

  8. Cidade Universitária, Castelo Branco, Universidade Federal da Paraíba, João Pessoa, Paraiba, 58051-900, Brazil

    Panmella P. Maciel

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  1. Ésio de C. Coelho Junior
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Correspondence to Panmella P. Maciel.

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de C. Coelho Junior, É., Maciel, P.P., de A. F. Muniz, I. et al. Poloxamer 407/chitosan micelles can improve α-Tocopherol effect on oral keratinocytes proliferation. Journal of Materials Research 36, 1447–1455 (2021). https://doi.org/10.1557/s43578-021-00169-7

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