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“Green” electrospinning of a collagen/hydroxyapatite composite nanofibrous scaffold

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Abstract

In this work, a composite nanofibrous scaffold of collagen/hydroxyapatite was prepared by electrospinning using a mild solvent. Hydroxyapatite particles dispersed into a collagen/acetic acid/water solution was electrospun to yield composite nanofibers. Scanning electron microscopy reveals nanofibers with an average diameter of 342 ± 67 nm, and a rough surface caused by the hydroxyapatite particles. Both X-ray and infrared spectroscopy confirmed the presence of the hydroxyapatite particles embedded in the collagen fibers. The inclusion of hydroxyapatite particles does not alter the native collagen structure. Lastly, these composite nanofibers support pre-osteoblast adhesion. These results show how "green" electrospinning could be used to generate nanocomposite scaffolds with potential biomedical applications.

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Acknowledgment

This work was financially supported by the NSF’s Puerto Rico Institute for Functional Nanomaterials (EPS-1002410), by the Puerto Rico Research, Science, and Technology Trust (Agreement Number 2016-00067), and by the “Programa de Apoyo Institucional Para la Formation en Estudios de Posgrados en Maestrias y Doctorados de La Universidad del Atlántico, Colombia” by providing DCC a scholarship. The authors thank Dr. Ricky Valentin for access to the electrospinning equipment; Dr. David Suleiman for access to the FTIR apparatus; Christian Rivera for assistance using the EDAX equipment; Beatriz Quinones for her support in the use of the FTIR; Ana M. Reyes for her support with the Particle Size Apparatus; Edwin Burgos, Coral Irizarry, & the UPRM Center for Biomedical Engineering and Nanomedicine for support of the in vitro experiments; and José Almodovar for providing assistance with the confocal microscope. The authors thank Dr. Anibal Quintana from Integra Lifesciences for generously donating collagen.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico, 00681-9000, USA

    David A. Castilla-Casadiego

  2. Department of Mechanical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico, 00681-9000, USA

    Michael Maldonado & Paul Sundaram

  3. Department of Chemical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico, 00681-9000, USA

    Jorge Almodovar

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  1. David A. Castilla-Casadiego
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  2. Michael Maldonado
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  4. Jorge Almodovar
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Correspondence to Jorge Almodovar.

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Castilla-Casadiego, D.A., Maldonado, M., Sundaram, P. et al. “Green” electrospinning of a collagen/hydroxyapatite composite nanofibrous scaffold. MRS Communications 6, 402–407 (2016). https://doi.org/10.1557/mrc.2016.43

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