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High-Performance Thin-Layer Chromatographic Method for Screening Antioxidant Compounds and Discrimination of Hibiscus sabdariffa L. by Principal Component Analysis

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Summary

The leaves of Hibiscus sabdariffa L. are one of the sources of food and traditional medicine. A combination of high-performance thin-layer chromatography (HPTLC) bioautographic assay with mass spectrometry (MS) has been performed to screen and identify the antioxidant compounds in the leaves of H. sabdariffa L. The crude extract of H. sabdariffa L. was separated on silica gel 60 HPTLC plates in an automatic develo** chamber (ADC2) with toluene-ethyl acetate-formic acid-methanol (6:6:1.6:1, v/v) as the mobile phase. Antioxidant bands were visualized by dip** in 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent. Five antioxidant compounds were identified as neochlorogenic acid (1), chlorogenic acid (2), cryptochlorogenic acid (3), rutin (4), and isoquercitrin (5), which could be the predominant contributors to the antioxidant activity of the leaves of H. sabdariffa L. Furthermore, principal component analysis (PCA) was carried out to discriminate ten accessions of H. sabdariffa L. using an image-processing software. This simple HPTLC fingerprint assisted by PCA can be used as a reliable method for the discrimination of different accessions of H. sabdariffa L.

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Authors and Affiliations

  1. SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wang**g, Chaoyang District, Bei**g, 100102, China

    ** Wang, **anshuang Cao & Feng Tang

  2. Urban Forestry Program, College of Science and Agriculture, Southern University, Baton Rouge, LA, 70813, United States

    Yadong Qi, Vanessa Ferchaud & Kit L. Chin

  3. Southern University Agricultural Research and Extension Center, Baton Rouge, LA, 70813, United States

    Yadong Qi, Vanessa Ferchaud & Kit L. Chin

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  1. ** Wang
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  2. **anshuang Cao
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  3. Yadong Qi
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  4. Vanessa Ferchaud
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  5. Kit L. Chin
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  6. Feng Tang
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Correspondence to Yadong Qi or Feng Tang.

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Wang, J., Cao, X., Qi, Y. et al. High-Performance Thin-Layer Chromatographic Method for Screening Antioxidant Compounds and Discrimination of Hibiscus sabdariffa L. by Principal Component Analysis. JPC-J Planar Chromat 28, 274–279 (2015). https://doi.org/10.1556/1006.2015.28.4.2

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  • DOI: https://doi.org/10.1556/1006.2015.28.4.2

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