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Effect of expression of manganese superoxide dismutase in baculovirus-infected insect cells

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Abstract

It has previously been demonstrated that baculovirus infection of the Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines leads to oxidative stress as measured by protein and membrane lipid oxidation and that this oxidative damage contributes to cell death. As a result of these findings, it was hypothesized that baculovirus infection stimulates superoxide radical (O ·—2 synthesis in the mitochondria and that the resulting O ·—2 accumulation overwhelms the cells’ antioxidant defenses. We investigated the ability of manganese superoxide dismutase (MnSOD) expression (which reduces O ·—2 to H2O2) to overcome the oxidative damage caused by baculovirus infection. It was found that MnSOD expression significantly reduced oxidative damage in baculovirus-infected Tn-5B1-4 cells but had no significant effect on oxidative damage in baculovirus-infected Sf-9 cells. The results are consistent with the hypothesis that O ·—2 accumulation in the mitochondria is at least partially responsible for the oxidative damage resulting from the baculovirus infection of insect cells.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, The University of Iowa, 52242, Iowa, City, IA

    Ying Wang, Gaurav Chauhan & David W. Murhammer

  2. Free Radical and Radiation Biology Program, The University of Iowa, 52242, Iowa City, IA

    Larry W. Oberley

  3. Department of Molecular Biology, University of Wyoming, PO Box 3944, 82071, Laramie, WY

    Dale Howe & Donald L. Jarvis

Authors
  1. Ying Wang
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  2. Larry W. Oberley
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  3. Dale Howe
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  4. Donald L. Jarvis
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  5. Gaurav Chauhan
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  6. David W. Murhammer
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Corresponding author

Correspondence to David W. Murhammer.

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Wang, Y., Oberley, L.W., Howe, D. et al. Effect of expression of manganese superoxide dismutase in baculovirus-infected insect cells. Appl Biochem Biotechnol 119, 181–193 (2004). https://doi.org/10.1385/ABAB:119:2:181

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  • DOI: https://doi.org/10.1385/ABAB:119:2:181

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