Abstract
The objective of this research was to establish a simple, practical and efficient method for routine quantitative analysis of Erigeron breviscapus and its extract injection to control their qualities. The reversed phase high performance liquid chromatographic method was adopted to determine simultaneously the contents of two major classes of constituents namely phenolic acids and flavonoids, which were usually ignored in previous studies of E. breviscapus. Under the optimum conditions, three flavonoids including scutellarin, scutellarein and apigenin and four phenolic acids including caffeic acid, chlorogenic acid, 3,4-O-dicaffeoylquinic acid and 3,5-O-dicaffeoylquinic acid were successfully separated on a Zorbax SB-C18 column (250 × 4.6 mm I.D., 5.0 μm particle size) at 25 °C. Of the three flavonoids, scutellatin is a flavone glucuronide. The mobile phase was a mixture of acetonitrile and 1.0% (v/v) aqueous acetic acid employing gradient elution at a flow rate of 1.0 mL min−1 and the detection wavelength was set at 330 nm. Regression equations revealed good linear relationship between the peak areas of the analytes and their concentrations (r 2> 0.9990). The relative standard deviations of retention time and peak area were less than 0.33 and 1.45%. The intra- and inter-day precisions as determined from sample solutions were below 1.66 and 2.35%. And the recoveries ranged from 96.5 to 101.8%. The proposed method has been successfully applied to the simultaneous quantification of two major classes of constituents in E. breviscapus and its extract injection for the first time.
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References
Editor Committee of Jiangsu New Medicine College (1995) Encyclopedia of traditional chinese medicine. Shanghai Science and Technology Press, Shanghai, pp. 948–949
Li SW, He P, Li CY (1999) Subtrop Plant Res Commun 28:5254
Prochaska HJ, Santamaria AB, Talalay P (1992) Proc Natl Acad Sci USA 89:2394–2398
Gali HU, Perchellet EM, Perchellet JP (1991) Cancer Res 51:2820–2825
Zhang DC, ** JQ, Liu XJ, Li JC, Wang JL (1985) Chin Tradit Herb Drugs 16:408–410
Guan BQ (1980) Chin Tradit Herb Drugs 11:480–482
Qu J, Wang YM, Luo GA (2000) Acta Pharm Sin 35:139–142
Yang WY, Zhang Y, Duan JG (2001) Chengdu Univ Tradit Chin Med 24:37–39
Zhang SX, Niu YJ, Lv HR, Ju XL (2002) Chin Trad Patent Med 24:95–97
Zou W, He GH, Wei DF (2003) Tradit Chin Drug Res Clin Pharm 14:190–192
Subramanian SS, Nair AGR (1972) Phytochemistry 11:439–440
Masayoshi O, Nizam UK, Nobusuke K (1975) J Chem Soc Perkin Trans 1:1563–1568
**ao HA (1981) Chin Tradit Herb Drugs 12:21–22
Liu H, Yang XL, Zhou LZ (2002) J Chin Med Mater 25:491–494
Zhang J, Li XS, Zhang WD (2002) Pharm Pract 20:103–107
Huang HB, Bao WF, Yang FF, Jia Y, Li K (2001) J Shenyang Pharm Univ 18:266–267, 293
Yue JM, Lin ZW, Wang DZ, Sun HD (1994) Phytochemistry 36:717–719
Hua J, Chen C, Xu Z (2002) Strait Pharm J 14:30–31
Qu J, Wang YM, Luo GA, Wu ZP (2001) J Chromatogr A 928:155–162
Qu J, Wang YM, Luo GA (2001) J Chromatogr A 919:437–441
Chu QC, Wu T, Fu L, Ye JN (2005) J Pharm Bio Ana 37:535–541
Yue JM, Zhao QS, Lin ZW (2000) Acta Bot Sin 42:311–313
Zhang RW, Zhang YL, Wang JS (1988) Chin Tradit Herb Drugs 19:729–731
Zhang WD, Kong DY, Li HT (1998) Chin J Pharm 29:554–555
Islam MS, Yoshimoto M, Yahara S (2002) J Agric Food Chem 50:3718–3722
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Wang, LW., Li, HJ., Li, P. et al. Simultaneous Quantification of Two Major Classes of Constituents in Erigeron breviscapus and Its Extract Injection by RP-HPLC. Chroma 66, 395–399 (2007). https://doi.org/10.1365/s10337-007-0320-9
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DOI: https://doi.org/10.1365/s10337-007-0320-9