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Differential Gene Expression Profiling in HELLP Syndrome Placentas

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Abstract

The aim of this study was to identify differentially expressed genes by suppression subtractive hybridization (SSH) in HELLP placentas. Two cDNA libraries were constructed; HSI (HELLP subtracted induced or upregulated) and HSS (HELLP subtracted suppressed or downregulated). Two hundred eighty-eight cDNA clones were sequenced; 37 were matched to GenBank entries and included genes in cell communication and organization, cellular processes, genetic information processing, and metabolic processes. A subgroup of 11 genes of interest was further selected for real-time quantitative polymerase chain reaction confirmation. Results showed no differences in expression of chosen upregulated genes between HELLP and non-HELLP placentas; 6 HELLP downregulated genes were significantly suppressed. Two genes related to production of secreted proteins, CTHRC1 and SERPINE2. SERPINE2 (PAI-1) is a soluble protease inhibitor and is a potential biomarker by Western blot analysis, and the protein is significantly decreased in HELLP placentas. SERPINE2 might be tested clinically in patients for early diagnosis of HELLP syndrome.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

    Bum-Yong Kang PhD, Stephen Tsoi PhD, Shan Zhu MD, Shenghui Su MD & Helen H. Kay MD

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  1. Bum-Yong Kang PhD
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  2. Stephen Tsoi PhD
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  3. Shan Zhu MD
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  4. Shenghui Su MD
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  5. Helen H. Kay MD
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Corresponding author

Correspondence to Helen H. Kay MD.

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The authors wish to thank Dr E. Albert Reece for his support of this work.

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Kang, BY., Tsoi, S., Zhu, S. et al. Differential Gene Expression Profiling in HELLP Syndrome Placentas. Reprod. Sci. 15, 285–294 (2008). https://doi.org/10.1177/1933719108314626

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