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Species and Intraspecies Level Diversity of Beauveria in Boreal Forests of Northwestern Russia

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Abstract—The diversity of entomopathogenic fungi, including, inter alia, members of the genus Beauveria, has been assessed insufficiently in Northwestern Russia. With respect to the contemporary taxonomical changes based on molecular markers, the available data are poor and need to be improved. We have established the species and intraspecies diversity of sixty Beauveria spp. isolates obtained from dead insects collected in Leningrad (2017), Novgorod (2018), Vologda (2018), and Pskov (2013, 2018) oblasts and in the Republic of Karelia (2017). Most fungi were isolated from beetles of the subfamily Scolytinae. Beauveria pseudobassiana was the predominant species in the studied subset (with an occurrence of 75.0%). B. bassiana (13.3%) and B. caledonica (11.7%) were isolated in smaller quantities. The latter species was considered rare for Russia; only one finding has been registered up to this time. Molecular analysis of the Bloc and tef1α loci allowed the designation of a new broad clade within B. pseudobassiana, comprised of haplotypes that had not been published in the nucleotide sequence databases previously. All other clades of the found species have a broad geographical distribution.

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Notes

  1. The values obtained with the BLASTn algorithm when comparing the query sequences with the reference sequences in the GenBank. The first value (query cover) is the percentage of the query sequence that overlaps the reference sequence; the second value (percent identity) denotes the percentage of similarity between the query and reference sequences in the alignment region.

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ACKNOWLEDGMENTS

The authors thank S.M. Malysh, M.N. Sabitova, and S.G. Udalov, researchers of the All-Russian Institute of Plant Protection, for their assistance in the study, as well as the reviewers for thorough analysis of the manuscript and valuable comments.

Funding

The study was supported by the Russian Foundation for Basic Research, project no. 17-04-00474 “Biodiversity and Functional Role of Mycobiota Associated with European Spruce Bark Beetle in the Boreal Forests of Northwest Russia,” as well as by the state task of the All-Russian Institute of Plant Protection.

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  1. All-Russian Institute of Plant Protection, 196608, St. Petersburg, Russia

    I. A. Kazartsev, G. R. Lednev & M. V. Levchenko

  2. Saint Petersburg State Forest Technical University, 194021, St. Petersburg, Russia

    I. A. Kazartsev

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Kazartsev, I.A., Lednev, G.R. & Levchenko, M.V. Species and Intraspecies Level Diversity of Beauveria in Boreal Forests of Northwestern Russia. Biol Bull Rev 13 (Suppl 1), S39–S49 (2023). https://doi.org/10.1134/S207908642307006X

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