Abstract
The work estimates histological changes in the myocardial structure of old, hypertensive rats after the introduction of cryopreserved cord-blood nucleated cells (CBNCs). The goal of the work was to estimate the structural organization of myocardial tissues and blood vessels on the third, seventh, and 30th days after the intraperitoneal administration of cryopreserved CBNCs to rats with stress-induced arterial hypertension. The experiments were carried out on 28- to 30-month-old, white, outbred, male rats. It was established that the introduction of cryopreserved CBNCs to old animals with stress-induced arterial hypertension led to a significant improvement in the structure of myocardial tissues and blood vessels. The number and area of cardiomyocyte nuclei increased already on the third day, and these changes were accompanied by a pronounced neoangiogenesis. The density of fibroblasts in the interstitial connective tissue and around the blood vessels increased by the 30th day after the introduction of cryopreserved CBNCs. There was a decrease in perivascular and interstitial edema, and there were no traits of focal ischemia and cardiosclerosis in the tissues.
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Funding
This work was performed within the framework of the departmental topic of the Department of Cryophysiology of the Institute for Problems of Cryobiology and Cryomedicine (National Academy of Sciences of Ukraine) “Formation of Adaptive Responses of the Organism of Experimental Animals in Conditions of Adaptation of Artificial Cooling and Cryopreserved Cord-Blood Nucleated Cells with Aging and Pathological Conditions” (cipher 2.2.6.103, state registration no. 0116U003493).
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Statement on animal welfare. The experiments were carried out in accordance with “General Principles of Working on Animals,” which was approved by the First National Congress of Bioethics (Kyiv, Ukraine, 2001) and is consistent with “European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes” (Strasbourg, France, 1986).
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Babiychuk, L.V., Koval, S.M., Babiychuk, V.G. et al. Histological Changes in the Myocardial Structure of Old Hypertensive Rats under the Influence of Cryopreserved Nucleated Cells from Umbilical Cord Blood. Adv Gerontol 11, 64–69 (2021). https://doi.org/10.1134/S2079057021010343
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DOI: https://doi.org/10.1134/S2079057021010343