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Binase penetration into alveolar epithelial cells does not induce cell death

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Abstract

Microbial ribonucleases possess a broad spectrum of biological activities, which demonstrate stimulating properties at low concentrations and cytotoxicity and genotoxicity at high concentrations. Mechanisms of their penetration into the cells still remain unclear. In this study penetration of Bacillus intermedius RNase (binase) in alveolar lung epithelial cells, type II (ATII) pneumocytes, has been investigated. Using immunofluorescence analysis we have shown for the first time internalization of binase by primary non-differentiated pneumocytes ATII. The enzyme did not penetrate in MLE-12 (Murine Lung Epithelial-12 cells). However, binase was cytotoxic towards tumor MLE-12 cells, but not ATII cells. These results clearly indicate higher sensitivity of tumor cells to binase compared to normal cells; they also demonstrate that penetration of the enzyme into alveolar epithelial cells is not directly associated with their death.

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References

  1. Makarov, A.A., Kolchinsky, A., and Ilinskaya, O.N., BioEssays, 2008, vol. 30, pp. 781–790.

    Article  CAS  Google Scholar 

  2. Leland, P. and Raines, R., Chemistry and Biology, 2001, vol. 8, pp. 405–413.

    Article  CAS  Google Scholar 

  3. Spalletti-Cernia, D., Sorrentino, S., Gaetano, S. Di Piccoli, R., Santoro, V., D’Alessio, G., Laccetti, P., and Vacchio, G., Brit. J. Cancer. Res., 2004, vol. 90, pp. 270–277.

    Article  CAS  Google Scholar 

  4. Ilinskaya, O.N. and Makarov, A.A., Mol. Biol. (Moscow), 2005, vol. 39, no. 1, pp. 1–11.

    Article  CAS  Google Scholar 

  5. Ardelt, W., Shogen, K., and Darzynkiewicz, Z., Curr. Pharm. Biotechnol., 2008, vol. 9, pp. 215–225.

    Article  CAS  Google Scholar 

  6. Sevcik, J., Urbanikova, L., Leland, P.A., and Raines, R.T., J. Biol. Chem., 2002, vol. 277, pp. 47325–47330.

    Article  CAS  Google Scholar 

  7. Mitkevich, V.A., Tchurikov, N.A., Zelenikhin, P.V., Petrushanko, I.Yu., Makarov, A.A., and Ilinskaya, O.N., FEBS Journal, 2010, vol. 277, pp. 186–196.

    Article  CAS  Google Scholar 

  8. Dobbs, L.G., Gonzalez, R., Matthay, M.A., Carter, E.P., Allen, L., and Verkman, A.S., Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 2991–2996.

    Article  CAS  Google Scholar 

  9. Chen, J., Chen, Z., Chintagari, N.R., Bhaskaran, M., **, N., Narasaraju, T., and Liu, L., J. Physiol., 2006, vol. 572, pp. 625–638.

    CAS  Google Scholar 

  10. Swain, R.J., Kemp, S.J., Goldstraw, P., Tetley, T.D., and Stevens, M.M., Biophys. J., 2010, vol. 98, pp. 1703–1711.

    Article  CAS  Google Scholar 

  11. Wikenheiser, K.A., Vorbroker, D.K., Rice, W.R., Clark, J.C., Bachurski, C.J., Oie, H.K., and Whitsett, J.A., Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 11029–11033.

    Article  CAS  Google Scholar 

  12. Schulga, A., Kurbanov, F., Kirpichnikov, M., Protasevich, I., Lobachev, V., Ranjbar, B., Chekhov, V., Polyakov, K., Engelborghs, Y., and Makarov, A., Protein Engineering, 1998, vol. 11, pp. 773–780.

    Google Scholar 

  13. Yakovlev, G.I., Moiseyev, G.P., Struminskaya, N.K., Borzykh, O.A., Kipenskaya, L.V., Znamenskaya, L.V., and Leschinskaya, I.B., FEBS Lett., 1994, vol. 354, pp. 305–306.

    Article  CAS  Google Scholar 

  14. Ilinskaya, O.N., Ivanchenko, O.B., Karamova, N.S., and Kipenskaya, L.V., Mut. Res., 1996, vol. 354, pp. 203–209.

    Article  Google Scholar 

  15. Ilinskaya, O.N., Zelenikhin, P.V., Petrushanko, I.Yu., Mitkevich, V.A., Prasolov, V.S., and Makarov, A.A., BBRC, 2007, vol. 361, pp. 1000–1005.

    CAS  Google Scholar 

  16. Mitkevich, V.A., Petrushanko, I.Yu., Kretova, O.V., Spirin, P.V., Zelenikhin, P.V., Prassolov, V.S., Tchurikov, N.A., Ilinskaya, O.N., Makarov A.A., Cell Cycle, 2010, vol. 9, pp. 2674–2678.

    Article  CAS  Google Scholar 

  17. Ilinskaya, O.N., Decker, K., Koschinski, A., Dreyer, F., and Repp, H., Toxicology, 2001, vol. 156, pp. 101–107.

    Article  CAS  Google Scholar 

  18. Cabrera Fuentes, H.A., Zelenikhin P.V., Kolpakov, A.I., Preissner, K.T., and Ilinskaya, O.N., Uch. Zap. Kazanskogo Universiteta, 2010, vol. 152, pp. 143–148.

    Google Scholar 

  19. Bocchetta, M., Di Resta, I., Powers, A., Fresco, R., Tosolini, A., Testa, J.R., Pass, H.I., Rizzo, P., and Carbone, M., Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 10214–10219.

    Article  CAS  Google Scholar 

  20. Bocchetta, M., Eliasz, S., De Marco, M.A., Rudzinski, J., Zhang, L., and Carbone, M., Cancer Res., 2008, vol. 68, pp. 1022–1029.

    Article  CAS  Google Scholar 

  21. Ali, S.H. and DeCaprio, J.A., Semin Cancer Biol., 2001, vol. 11, no. 1, pp. 15–23.

    Article  CAS  Google Scholar 

  22. Cheniclet, C. and Bendayan, M., J. Histochem. Cytochem., 1990, vol. 38, pp. 551–562.

    Article  CAS  Google Scholar 

  23. Carroll, R.B., Samad, A., Mann, A., Harper, J., and Anderson, C.W., Oncogene, 1988, vol. 2, pp. 437–444.

    CAS  Google Scholar 

  24. Abraham, A.T., Lin, J., Newton, D.L., Rybak, S., and Hecht, S., Chem. Biol., 2003, vol. 10, pp. 45–52.

    Article  CAS  Google Scholar 

  25. Lee, V.Y., Schroedl, C., Brunelle, J.K., Buccellato, L.J., Akinci, O.I., Kaneto, H., Snyder, C., Eisenbart, J., Budinger, S., and Chandel, N.S., Am. J. Physiol. Lung. Cell Mol. Physiol., 2005, vol. 289, pp. 521–528.

    Article  Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany

    H. A. Cabrera-Fuentes, R. T. Mukhametshina & G. Barreto

  2. Kazan Federal University, ul. Kremlevskaya 18, Kazan, 420008, Russia

    H. A. Cabrera-Fuentes, N. V. Kalacheva, P. V. Zelenikhin, A. I. Kolpakov & O. N. Ilinskaya

  3. Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany

    K. T. Preissner

Authors
  1. H. A. Cabrera-Fuentes
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  2. N. V. Kalacheva
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  3. R. T. Mukhametshina
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  4. P. V. Zelenikhin
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  5. A. I. Kolpakov
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  6. G. Barreto
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  7. K. T. Preissner
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  8. O. N. Ilinskaya
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Corresponding author

Correspondence to P. V. Zelenikhin.

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Original Russian Text © H.A. Cabrera-Fuentes, N.V. Kalacheva, R.T. Mukhametshina, P.V. Zelenikhin, A.I. Kolpakov, G. Barreto, K.T. Preissner, O.N. Ilinskaya, 2012, published in Biomeditsinskaya Khimiya.

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Cabrera-Fuentes, H.A., Kalacheva, N.V., Mukhametshina, R.T. et al. Binase penetration into alveolar epithelial cells does not induce cell death. Biochem. Moscow Suppl. Ser. B 6, 317–321 (2012). https://doi.org/10.1134/S199075081204004X

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  • DOI: https://doi.org/10.1134/S199075081204004X

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