Abstract
meso-CF3-Fluorophores BODIPY were obtained using a methodology including, as a key step, the condensation of trifluoro(pyrrolyl)ethanols with pyrroles having aryl or heterocyclic substituents. The resulting compounds were studied as lysosome-specific fluorescent dyes on Ehrlich carcinoma cells. It was found that a bright fluorescence of cells in the red region of the spectrum is observed for four (1a and 1c–1e) of the five studied fluorophores. Fluorophore 1a is capable of selective accumulation in lysosomes and can be used for intravital monitoring of intracellular processes. Fluorophores 1c–1e also exhibit a bright fluorescence in the blue and green regions of the spectrum.
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ACKNOWLEDGMENTS
The study was carried out using the equipment of the Center for Shared Use “Diagnostic Images in Surgery.”
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The work was carried out with the support of research plans in the State Register of the Institute of Chemical Sciences of the Russian Academy of Sciences no. 121021000199-6 and ISCST no. 122022200212-6.
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The study protocol was approved by the ethics committee of the Irkutsk Scientific Center of Surgery and Traumatology. The authors declare no conflict of interest.
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Shurygina, I.A., Dremina, N.N., Trukhan, I.S. et al. meso-CF3-Difluoroboradiazaindacenes (BODIPY) as Markers for Visualization of Lysosomes. Russ J Gen Chem 93 (Suppl 1), S173–S179 (2023). https://doi.org/10.1134/S1070363223140414
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DOI: https://doi.org/10.1134/S1070363223140414