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Development and Validation of the HPLC-DAD Method for the Simultaneous Determination of Anthraquinones in Rumex crispus L. and Rumex acetosella L. Plants and Evaluation of Their Antioxidant Capacities

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Abstract

In this study, an HPLC-DAD method with high separation efficiency, detection sensitivity, and selectivity was developed and validated for the simultaneous qualitative and quantitative determination of anthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion) in Rumex acetosella L. and Rumex crispus L. plants collected at different regions and times. Optimal method conditions were determined by studying different mobile phases, mobile phase compositions, flow rates, column, column temperatures, injection volume, and wavelength of the compounds. Validation studies were carried out to prove the validity of the developed method. The correlation coefficients for all compounds were found to be in the range of 0.9991–0.9997, and the calibration curves were linear in the concentration range of 0.1–1.0 μg/mL. The limits of detection and quantification values were calculated in the range of 0.014–0.017 and 0.017–0.057 μg/mL, respectively. The developed and validated method indicated that it is effective and suitable for determining aloe-emodin, rhein, emodin, chrysophanol, and physcion in extracts of Rumex plants. In addition, the antioxidant activities of the plant extracts were determined using the Cupric Ion Reducing Antioxidant Capacity and 2,2-diphenyl-1-picrylhydrazil radical assays, revealing their antioxidant properties.

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ACKNOWLEDGMENTS

The authors are thankful to Professor Şükran Kültür for identifying the plant material and voucher specimen.

Funding

This study was funded by Scientific Research Projects Coordination Unit of Istanbul University. Project number: FBA-2019-33300.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134, Istanbul, Turkey

    Gizem Yıldırım Baştemur & Sabriye Perçin Özkorucuklu

  2. Programme of Molecular Biotechnology and Genetics, Institute of Graduate Studies in Sciences, Istanbul University, 34116, Istanbul, Turkey

    Reyhan Akpınar

  3. Department of Chemistry, Faculty of Art and Science, Süleyman Demirel University, 32260, Isparta, Turkey

    Esengül Kır

Authors
  1. Gizem Yıldırım Baştemur
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  2. Reyhan Akpınar
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  3. Esengül Kır
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Correspondence to Gizem Yıldırım Baştemur.

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Baştemur, G., Akpınar, R., Kır, E. et al. Development and Validation of the HPLC-DAD Method for the Simultaneous Determination of Anthraquinones in Rumex crispus L. and Rumex acetosella L. Plants and Evaluation of Their Antioxidant Capacities. J Anal Chem 79, 180–190 (2024). https://doi.org/10.1134/S1061934824020035

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  • DOI: https://doi.org/10.1134/S1061934824020035

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