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Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds

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Abstract

The retention profile of six serotonin receptor ligands such as arylpiperazines (aripiprazole, ziprasidone, risperidone), and benzothiazepine derivatives (olanzapine, mianserin, quetiapine), was investigated on C8 alkyl and pentafluorophenylpropyl stationary phases. The experimental design methodology was used to define their retention behavior and achieve acceptable separation results. Both phases showed satisfactory selectivity for all compounds. The optimal separation among the structurally related arylpiperazines and benzothiazepines was found with the C8 phase at 25°C, using acetonitrile (40%, v/v) and 20 mM ammonium acetate as a mobile phase modifier. The selectivity and sensitivity performances of the selected C8 system were discussed considering the separation of aripiprazole and its related compounds. In addition, the specified conditions were validated for determining aripiprazole in pharmaceutical dosage forms. The developed reversed-phase high-performance liquid chromatography method can be successfully used for the rapid chromatographic profiling of serotonin receptor ligands and related analogs, providing increased selectivity during pharmaceutical analysis.

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Funding

The authors acknowledge the funding provided by the Institute of Physics Belgrade, through the grant from the Ministry of Science, Technological Development, and Innovation of the Republic of Serbia. The part of research was also funded by the Ministry of Science, Technological Development and Innovation, Republic of Serbia, through a Grant Agreement with University of Belgrade, Faculty of Pharmacy (no: 451-03-47/2023-01/200161).

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Authors and Affiliations

  1. Institute of Physics Belgrade, 11080, Belgrade, Serbia

    Darija Obradović & Saša Lazović

  2. Department of Pharmaceutical Chemistry, University of Belgrade, Faculty of Pharmacy, 11000, Belgrade, Serbia

    Jelena Savić, Jovana Joksimović, Bojan Marković & Zorica Vujić

Authors
  1. Darija Obradović
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  2. Jelena Savić
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  3. Jovana Joksimović
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  6. Saša Lazović
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Correspondence to Darija Obradović.

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Obradović, D., Savić, J., Joksimović, J. et al. Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds. J Anal Chem 79, 95–104 (2024). https://doi.org/10.1134/S1061934824010076

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