Abstract
Conditions for the formation of a recognition layer of a piezoelectric immunosensor based on magnetic carbon nanocomposites (MCNCs) under the action of an external magnetic field are studied. The effects of the size and number of magnetic nanoparticles (MNPs) in the composite on the analytical characteristics of the gravimetric immunosensor are revealed. Scanning electron microscopy is used to determine the average sizes of Fe3O4 magnetic nanoparticles synthesized by coprecipitation. It is noted that the minimum weight and stability of the recognition layer were observed for the nanocomposite obtained at a ratio of carbon nanotubes and MNPs with an average diameter of 22 nm equal to 3 : 1. The formation of peptide bonds between the MCNCs and a penicillin G conjugate was established by IR spectrometry. It was shown that the use of magnetic carbon nanocomposites in the formation of a recognition layer makes it possible to significantly simplify the procedure for preparing a piezoelectric sensor for analysis and reduce its duration from 24 to 1.5 h. The range of the determined antibiotic concentrations is 1–450 ng/mL, the limit of detection is 0.5 ng/mL.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1134%2FS1061934823040068/MediaObjects/10809_2023_1923_Fig1_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1134%2FS1061934823040068/MediaObjects/10809_2023_1923_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1134%2FS1061934823040068/MediaObjects/10809_2023_1923_Fig3_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1134%2FS1061934823040068/MediaObjects/10809_2023_1923_Fig4_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1134%2FS1061934823040068/MediaObjects/10809_2023_1923_Fig5_HTML.png)
REFERENCES
Gupta, B.K., Yadav, A., Koch, P., and Mishra, P., in Biosensors in Food Safety and Quality, Mishra, P. and Sahu, P.P., Eds., Boca Raton: CRC, 2022, p. 37.
Zhang, J., Zhang, X., Wei, X., Xue, Y., Wan, H., and Wang, P., Anal. Chim. Acta, 2021, vol. 1164, p. 338321.
Guliy, O.I., Zaitsev, B.D., Alsowaidi A.K.M., Karavaeva, O.A., Lovtsova, L.G., and Borodina, I.A., Biophysics (Moscow), 2021, vol. 66, no. 4, p. 555.
Alsowaidi A.K.M., Karavaeva, O.A., and Guliy, O.I., Antibiot. Khimioter., 2022, vol. 67, nos. 1–2, p. 53.
Ermolaeva, T.N., Kalmykova, E.N., and Shashkanova, O.Yu., Russ. J. Gen. Chem., 2008, vol. 78, no. 12, p. 2430.
Immobilization Strategies: Biomedical, Bioengineering and Environmental Applications, Tripathi, A. and Melo, J.S., Eds., Singapore: Springer, 2021.
Karaseva, N.A. and Ermolaeva, T.N., Talanta, 2014, vol. 120, p. 312.
Pohanka, M., Materials, 2018, vol. 11, no. 3, p. 448.
Shukshina, E.I., Farafonova, O.V., Shanin, I.A., Grazhulene, S.S., Eremin, S.A., and Ermolaeva, T.N., Sorbtsionnye Khromatogr. Protsessy, 2018, vol. 18, no. 3, p. 394.
Tajyani, S. and Babaei, A., J. Electroanal. Chem., 2018, vol. 808, p. 50.
Santos, A.M., Wong, A., Prado, T.M., Fava, E.L., Fatibello-Filho, O., Sotomayor, M.D.P.T., and Moraes, F.C., Talanta, 2021, vol. 224, p. 121804.
Sohouli, E., Khosrowshahi, E.M., Radi, P., Naghian, E., Rahimi-Nasrabadi, M., and Ahmadi, F., J. Electroanal. Chem., 2020, vol. 877, p. 114503.
Reddy, K.R., Reddy, P.A., Reddy, C.V., Shetti, N.P., Babu, B., Ravindranadh, K., Shankar, M.V., Reddy, M.C., Soni, S., and Naveen, S., Methods Microbiol., 2019, vol. 46, p. 227.
Kouhpanji, M.R.Z. and Stadler, B.J.H., Sensors, 2020, vol. 20, no. 9, p. 2554.
Bayramoglu, G., Ozalp, V.C., Oztekin, M., and Arica, M.Y., Talanta, 2019, vol. 200, p. 263.
Pohanka, M., Chem. Pap., 2020, vol. 74, p. 451.
Wan, Y., Zhang, D., and Hou, B., Biosens. Bioelectron., 2010, vol. 25, p. 1847.
Bizina, E.V., Farafonova, O.V., Zolotareva, N.I., Grazhulene, S.S., and Ermolaeva, T.N., J. Anal. Chem., 2022, vol. 77, no. 4, p. 375.
Grazhulene, S.S., Zolotareva, N.I., Red’kin, A.N., Shilkina, N.N., Mitina, A.A., and Khodos, I.I., Russ. J. Appl. Chem., 2020, vol. 93, no. 1, p. 57.
Sauerbrey, G., Z. Phys., 1959, vol. 55, p. 206.
Grazhulene, S.S., Zolotareva, N.I., Red’kin, A.N., Shilkina, N.N., Mitina, A.A., and Kolesnikova, A.M., Russ. J. Appl. Chem., 2018, vol. 91, no. 11, p. 1849.
Wang, J., Zheng, S., Shao, Y., Liu, J., Xu, Z., and Zhu, D., J. Colloid Interface Sci., 2010, vol. 349, p. 293.
Singh, S., Barick, K.C., and Bahadur, D., J. Hazard. Mater., 2011, vol. 192, p. 1539.
Eguilaz, M., Villalonga, R., Yanez-Sedeno, P., and **arron, J.M., Anal. Chem., 2011, vol. 83, p. 7807.
Mikhaylova, M., Kim, D.K., Berry, C.C., Zagorodni, A., Toprak, M., Curtis, A.S.G., and Muhammed, M., Chem. Mater., 2004, vol. 16, no. 12, p. 2344.
Netto, C.G.C.M., Toma, H.E., and Andrade, L.H., J. Mol. Catal. B: Enzym., 2013, vol. 85, p. 71.
Nartova, Yu.V., Eremin, S.A., and Ermolaeva, T.N., J. Anal. Chem., 2008, vol. 63, no. 12, p. 1191.
Grazhulene, S.S., Zolotareva, N.I., Red’kin, A.N., Shilkina, N.N., Mitina, A.A., and Khodos, I.I., Russ. J. Appl. Chem., 2020, vol. 93, no. 1, p. 57.
Funding
This work was supported by the Russian Foundation for Basic Research and the Lipetsk Region, project no. 20-43-480001. At the Institute for Problems of Microelectronics Technology and High-Purity Materials of the Russian Academy of Sciences, the work was carried out within the framework of State Assignment 075-01304-23-00.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflicts of interest.
Additional information
Translated by V. Kudrinskaya
Rights and permissions
About this article
Cite this article
Bizina, E.V., Farafonova, O.V., Zolotareva, N.I. et al. Use of Magnetic Carbon Nanocomposites in the Formation of a Recognition Layer of a Piezoelectric Immunosensor for the Determination of Penicillin G. J Anal Chem 78, 488–496 (2023). https://doi.org/10.1134/S1061934823040068
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934823040068