Abstract
A piezoelectric immunosensor with a recognition layer based on magnetic carbon nanocomposites is developed for the determination of ciprofloxacin. The receptor coating of the sensor is formed by the action of a magnetic field on magnetic particles located on the surface of carbon nanotubes modified with a ciprofloxacin conjugate. The sizes of magnetic particles in the composition of the nanocomposite are determined by scanning electron microscopy. A dependence of the mass of the recognition coating on the size of magnetic particles on the surface of carbon nanotubes is shown. A detection cell with a sensor located above a neodymium magnet is proposed. The analytical characteristics of the immunosensor are determined, the limit of detection for ciprofloxacin is 2 ng/mL, and the linear range of determined concentrations is 5–400 ng/mL. The use of magnetic carbon nanocomposites in the creation of a recognition layer ensures the reduction of the time of sensor preparation to analysis from 24 to 1.5 h and extends its service life. The sensor is tested in the detection of antibiotics in milk and meat.
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Funding
This work was supported by the Russian Foundation for Basic Research and the Lipetsk Region, project no. 20-43-480001. At the Institute for Problems of Microelectronics Technology and High-Purity Materials of the Russian Academy of Sciences, the work was carried out within the framework of State Assignment no. 075-00355-21-0.
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Translated by V. Kudrinskaya
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Bizina, E.V., Farafonova, O.V., Zolotareva, N.I. et al. A Piezoelectric Immunosensor Based on Magnetic Carbon Nanocomposites for the Determination of Ciprofloxacin. J Anal Chem 77, 458–465 (2022). https://doi.org/10.1134/S1061934822040049
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DOI: https://doi.org/10.1134/S1061934822040049