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Identification of Hybrid Specimens of Rock Lizards Darevskia armeniaca × Darevskia valentini Based on Microsatellite Genoty**

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Abstract

The paper presents the results of the microsatellite genoty** of hybrid lizards D. armeniaca × D. valentini from sympatric populations of Armenia. Triploid individuals are identified based on the totality of the Du281 and Du47G loci and their origin is determined. It is shown that the hybrids contain both alleles of the maternal parthenospecies D. armeniaca and alleles of the paternal species D. valentini.

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REFERENCES

  1. Dobzhansky, Th., Genetic nature of species differences, Am. Nat., 1937, vol. 71, no. 735, pp. 404—420. https://doi.org/10.1086/280726

    Article  Google Scholar 

  2. Uetz, P., Freed, P., Aguilar, R., and Hošek, J., The Reptile Database. http://www.reptile-database.org. Accessed October 10, 2022.

  3. Danielayn, F., Arakelyan, M., and Stepanyan, I., The progress of microevolution in hybrids of rock lizards of genus Darevskia, Biol. Zh. Arm., 2008, vol. 60, nos. 1–2, pp. 147—156.

    Google Scholar 

  4. Spangenberg, V., Arakelyan, M., Galoyan, E., et al., Reticulate evolution of the rock lizards: meiotic chromosome dynamics and spermatogenesis in diploid and triploid males of the genus Darevskia, Genes, 2017, vol. 8, no. 6, p. 149. https://doi.org/10.3390/genes8060149

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Moritz, C., Wright, J.W., and Brown, C.M., Mitochondrial DNA analysis and the origin and relative age of parthenogenetic lizards (genus Cnemidophorus): III. C. velox and C. exsanguis, Evolution, 1989, vol. 43, no. 5, pp. 958—968. https://doi.org/10.1111/j.1558-5646.1989.tb02542.x

    Article  PubMed  Google Scholar 

  6. Taylor, H.L., Walker, J.M., Cole, C.J., and Dessauer, H.C., Morphological divergence and genetic variation in the triploid parthenogenetic teiid lizard, Aspidoscelis neotesselata, J. Herpetol., 2015, vol. 49, no. 3, pp. 491—501. https://doi.org/10.1670/14-057

    Article  Google Scholar 

  7. Araya-Donoso, R., Véliz, D., Vidal, M., and Lamborot, M., Relationships of the morphological variation in diploids, triploids and mosaics of Liolaemus chiliensis (Sauria: Liolaemidae), Amphibia—Reptilia, 2017, vol. 38, no. 4, pp. 503—515. https://doi.org/10.1163/15685381-00003132

    Article  Google Scholar 

  8. Kluge, A.G. and Eckardt, M.J., Hemidactylus garnotii Dumeril and Bibron, a triploid all-female species of gekkonid lizard, Copeia, 1969, no. 4, pp. 651—664. https://doi.org/10.2307/1441789

  9. Darevskii, I.S. and Kulikova, V.N., Taxonomic characters and certain peculiarities of the oogenesis of the hybrids between bisexual and parthenogenetic forms of the rock lizard, Tsitologiya, 1962, vol. 4, no. 2, pp. 160—170.

    CAS  Google Scholar 

  10. Darevsky, I.S. and Danielyan, F.D., Diploid and triploid progeny arising from natural mating of parthenogenetic Lacerta armeniaca and L. unisexualis with bisexual L. saxicola valentini, J. Herpetol., 1968, vol. 2, nos. 3–4, pp. 65—69. https://doi.org/10.2307/1563104

    Article  Google Scholar 

  11. Petrosyan, V.G., Osipov, F.A., Bobrov, V.V., et al., New records and geographic distribution of the sympatric zones of unisexual and bisexual rock lizards of the genus Darevskia in Armenia and adjacent territories, Biodiversity Data J., 2020, vol. 8, pp. 1—30. https://doi.org/10.3897/BDJ.8.e56030

    Article  Google Scholar 

  12. Arakelyan, M.S., Danielyan, F.D., Corti, C., et al., Herpetofauna of Armenia and Nagoro-Karabakh, Salt Lake City: Society for Study of Amphibians and Reptiles, 2011.

    Google Scholar 

  13. Freitas, S.N., Harris, D.J., Sillero, N., et al., The role of hybridization in the origin and evolutionary persistence of vertebrate parthenogens: a case study of Darevskia lizards, Heredity, 2019, vol. 123, no. 6, pp. 795—808. https://doi.org/10.1038/s41437-019-0256-5

    Article  PubMed  PubMed Central  Google Scholar 

  14. Carretero, M., García-Muñoz, E., Argaña, E., et al., Parthenogenetic Darevskia lizards mate frequently if they have the chance: a quantitative analysis of copulation marks in a sympatric zone, J. Nat. Hist., 2018, vol. 52, nos. 7—8, pp. 405—413. https://doi.org/10.1080/00222933.2018.1435832

    Article  Google Scholar 

  15. Galoyan, E., Bolshakova, A., Abrahamyan, M., et al., Natural history of Valentin’s rock lizard (Darevskia valentini) in Armenia, Zool. Res., 2019, vol. 40, no. 4, pp. 277—292. https://doi.org/10.24272/j.issn.2095-8137.2019.036

    Article  PubMed  PubMed Central  Google Scholar 

  16. Darevsky, I.S. and Danielyan, F.D., Natural allotriploidy in some rock lizards species of the Caucasus and the probable evolutionary significance of this phenomenon, Evolyutsiya, ekologiya, vidoobrazovanie (Evolution, Ecology, Speciation) (Proc. Conf. in Memoriam of N.N. Vorontsov (1934—2000)), Moscow: Uchebno-Nauchnyi Tsentr Dovuzovskogo Obrazovaniya, 2001, pp. 131—133.

  17. Girnyk, A.E., Vergun, A.A., Arakelyan, M.S., and Ryskov, A.P., Molecular genetic identification of Darevskia unisexualis × D. valentini triploid hybrids from sympatric populations of Armenia, Mol. Genet., Mikrobiol. Virusol., 2021, vol. 39, no. 3, pp. 46—48. https://doi.org/10.17116/molgen20213903146

    Article  Google Scholar 

  18. Girnyk, A.E., Vergun, A.A., Omelchenko, A.V. et al. Molecular and genetic characterization of the allelic variants of Du215, Du281, Du323, and Du47G microsatellite loci in parthenogenetic lizard Darevskia armeniaca (Lacertidae), Russ. J. Genet., 2017, vol. 53, no. 4, pp. 472—482. https://doi.org/10.1134/S1022795417040068

    Article  CAS  Google Scholar 

  19. Mathew, C.G.P., The isolation of high molecular weight eukaryotic DNA, in Nucleic Acids: Methods in Molecular Biology, Humana Press, 1984, vol. 2, pp. 31—34. https://doi.org/10.1385/0-89603-064-4:31.

  20. Korchagin, V.I., Badaeva, T.N., Tokarskaya, O.N., et al., Molecular characterization of allelic variants of (GATA)n microsatellite loci in parthenogenetic lizards Darevskia unisexualis (Lacertidae), Gene, 2007, vol. 392, pp. 126—133. https://doi.org/10.1016/j.gene.2006.11.020

    Article  CAS  PubMed  Google Scholar 

  21. Girnyk, A.E., Vergun, A.A., Semyenova, S.K., et al., Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia armeniaca, BMC Genomics, 2018, vol. 19, no. 1, pp. 1—12. https://doi.org/10.1186/s12864-018-5359-5

    Article  CAS  Google Scholar 

  22. Vergun, A.A., Girnyk, A.E., Korchagin, V.I., et al., Origin, clonal diversity, and evolution of the parthenogenetic lizard Darevskia unisexualis, BMC Genomics, 2020, vol. 21, no. 351, pp. 1—10. https://doi.org/10.1186/s12864-020-6759-x

    Article  CAS  Google Scholar 

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ACKNOWLEDGMENTS

We thank I.S. Darevsky for providing biological samples and I.A. Martirosyan for valuable advice concerning the preparation of this work.

Funding

This study was supported by the Russian Scientific Foundation, project no. 19-14-00083.

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Correspondence to A. E. Girnyk.

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Conflict of interest. The authors declare that they have no conflicts of interests.

Statement on the welfare of animals. The animals in this study were treated in accordance with the standards provided by the Ministry of Nature Protection of Armenia (permission no. 5/22.1/51043). The study was approved by the Ethical Committee of Moscow State University (permission no. 24-01) and was carried out completely in accordance with corresponding ethical principles and scientific standards.

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Translated by M. Bibov

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Girnyk, A.E., Vergun, A.A. & Ryskov, A.P. Identification of Hybrid Specimens of Rock Lizards Darevskia armeniaca × Darevskia valentini Based on Microsatellite Genoty**. Russ J Genet 59, 627–631 (2023). https://doi.org/10.1134/S1022795423060078

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