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Study of the Genotoxicity of Beta-Propiolactone Using Lux Biosensors E. coli and the Nematode Caenorhabditis elegans

  • GENERAL GENETICS
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Abstract

For the first time, lux biosensors of E. coli and the nematode Caenorhabditis elegans were used to study the genotoxicity of beta-propiolactone (BPL) used in the production of inactivated viral vaccines as an inactivator. It has been shown that the DNA-damaging activity of BPL is due not only to its ability to bind to bacterial DNA, but also to the ability to generate in the cell reactive oxygen species such as superoxide anions and peroxide, which have genotoxic activity. It was found that BPL in a dose-dependent manner, starting from a concentration of 0.001 mol/L, reduces the survival of bacteria. However, the intensity of expression of the antioxidant defense gene of superoxide dismutase soxS and the DNA repair gene colD increased. BPL-induced DNA breaks in nematode cells were detected by electrophoresis. The antioxidant acetylcysteine reduced the genotoxic effects of BPL on both bacteria and the nematode.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia

    E. A. Machigov, S. K. Abilev & E. V. Igonina

  2. Moscow State University, 119234, Moscow, Russia

    S. K. Abilev & M. V. Marsova

Authors
  1. E. A. Machigov
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Correspondence to S. K. Abilev.

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Machigov, E.A., Abilev, S.K., Igonina, E.V. et al. Study of the Genotoxicity of Beta-Propiolactone Using Lux Biosensors E. coli and the Nematode Caenorhabditis elegans. Russ J Genet 59, 432–440 (2023). https://doi.org/10.1134/S1022795423040075

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