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Analysis of Polymorphism of the Genes ADCY8 and RYR3 for Identification of Wild and Domestic Animals of the Species Canis lupus

  • ANIMAL GENETICS
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Abstract

As a result of the study, we confirmed the high differentiating potential of polymorphic variants g.27748425T>C (ADCY8 gene) and g.1414373T>C (RYR3 gene). A test model of two polymorphisms for the differentiation of a wolf and a domestic dog is proposed, which is distinguished by high values of accuracy (96.2%), specificity (96.3%), and sensitivity (98.9%). Using KASP (kompetitive allele-specific PCR), a quick and simple approach to differentiation based on the proposed test model has been developed, which is designed to reduce the time and cost of molecular genetic analysis, as well as reduce the risk of cross contamination, because the process is one-stage (restriction and electrophoresis stages are excluded).

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Funding

This study was carried out with partial financial support from Maksim Medical LLC as part of the private scientific initiative “Analysis of SRA Data Using Bioinformatics Methods.”

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Authors and Affiliations

  1. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, 220072, Minsk, Belarus

    V. N. Kipen, E. V. Snytkov & A. N. Viarchuk

  2. Maxim Medical LLC, 123423, Moscow, Russia

    M. M. Patrin

  3. International Sakharov Environmental Institute of Belarusian State University, 220070, Minsk, Belarus

    E. V. Snytkov

  4. Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 220073, Minsk, Belarus

    A. N. Viarchuk

  5. WellVet LLC, 220063, Minsk, Belarus

    A. N. Semak

Authors
  1. V. N. Kipen
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  2. M. M. Patrin
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  3. E. V. Snytkov
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  4. A. N. Viarchuk
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  5. A. N. Semak
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Correspondence to V. N. Kipen.

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Conflict of interest. The authors declare that they have no conflicts of interest.

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Kipen, V.N., Patrin, M.M., Snytkov, E.V. et al. Analysis of Polymorphism of the Genes ADCY8 and RYR3 for Identification of Wild and Domestic Animals of the Species Canis lupus. Russ J Genet 59, 289–296 (2023). https://doi.org/10.1134/S1022795423030067

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  • DOI: https://doi.org/10.1134/S1022795423030067

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