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Genetic cell reprogramming: A new technology for basic research and applied usage

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Abstract

Gene function disclosure and the development of modern technologies of genetic manipulations offered the possibility of genetic reprogramming application to alter cell specialization. With the involvement of a gene set that encodes the transcription factors responsible for the pluripotent state, any cell of an adult body could be reprogrammed into the embryonal state and pluripotency could be induced in this cell. Such reprogrammed cells were called induced pluripotent stem cells (iPSCs), and they are capable of again passing through all developmental stages. This provides new possibilities for studies of the basic mechanisms of developmental biology, the formation of specific cell types, and the whole body. In culture, iPSCs could be maintained permanently in a nontransformed state and permit genetic manipulations while maintaining their pluripotent properties. Such a unique combination of their properties makes them an attractive tool for studies of various pathologies and for the delineation of treatment approaches. This review discusses the basic and applied aspects of iPSCs biology.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    A. N. Bogomazova, E. M. Vassina, S. L. Kiselev, M. A. Lagarkova, O. S. Lebedeva, E. D. Nekrasov, A. V. Panova, E. S. Philonenko, E. A. Khomyakova, L. V. Tskhovrebova, I. V. Chestkov & M. V. Shutova

  2. Scoltech Center for Stem Cell Research, Moscow, 143026, Russia

    A. N. Bogomazova, E. M. Vassina, S. L. Kiselev, M. A. Lagarkova, E. D. Nekrasov, A. V. Panova, E. S. Philonenko, E. A. Khomyakova, I. V. Chestkov & M. V. Shutova

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  1. A. N. Bogomazova
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  2. E. M. Vassina
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  3. S. L. Kiselev
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  4. M. A. Lagarkova
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  6. E. D. Nekrasov
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  7. A. V. Panova
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  8. E. S. Philonenko
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  9. E. A. Khomyakova
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  12. M. V. Shutova
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Correspondence to S. L. Kiselev.

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Original Russian Text © A.N. Bogomazova, E.M. Vassina, S.L. Kiselev, M.A. Lagarkova, O.S. Lebedeva, E.D. Nekrasov, A.V. Panova, E.S. Philonenko, E.A. Khomyakova, L.V. Tskhovrebova, I.V. Chestkov, M.V. Shutova, 2015, published in Genetika, 2015, Vol. 51, No. 4, pp. 466–478.

All of the authors equally contributed to the writing of this paper.

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Bogomazova, A.N., Vassina, E.M., Kiselev, S.L. et al. Genetic cell reprogramming: A new technology for basic research and applied usage. Russ J Genet 51, 386–396 (2015). https://doi.org/10.1134/S102279541504002X

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