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Effect of Elevated Temperature on Premature Senescence in Nodules of Pea (Pisum sativum L.) sym26 and sym27 Mutants. II. Ultrastructural Organization

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Abstract

Delaying nodule senescence and prolonging the time of nitrogen fixation are promising objectives for increasing legume yields. However, the mechanisms of nodule senescence still require further investigation. In this study, the ultrastructural organization of symbiotic nodules subjected to the combined effect of two factors that activate premature senescence of symbiotic nodules: ineffective interaction and elevated temperature (28°C) was investigated. It was demonstrated that symbiotically ineffective pea (Pisum sativum L.) mutant lines SGEFix−-3 (sym26) and SGEFix−-7 (sym27) exhibiting a premature senescence phenotype respond differently to a stress factor such as elevated temperature. Indeed, the mutant line SGEFix−-3 (sym26) exhibited pronounced oxidative stress reactions, while the mutant line SGEFix−-7 (sym27) developed reactions affecting cell walls.

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ACKNOWLEDGMENTS

The research was performed using equipment of the Core Centrum «Genomic Technologies, Proteomics and Cell Biology» in ARRIAM.

Funding

This work was funded by the Russian Science Foundation, grant no. 21-16-00117.

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Authors and Affiliations

  1. All-Russia Research Institute for Agricultural Microbiology, St. Petersburg, Russia

    A. V. Tsyganova, E. V. Seliverstova, A. P. Gorshkov, T. A. Serova & V. E. Tsyganov

  2. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    E. V. Seliverstova

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  1. A. V. Tsyganova
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Contributions

V.E.T. conceptualized and administrated of the project, edited the manuscript. T.A.S. designed the experiments. A.P.G. and T.A.S. collected samples and performed the experiments. E.V.S. and A.V.T. performed an electron microscopic examination. A.V.T. drafted the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to A. V. Tsyganova.

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Abbreviations: ROS, reactive oxygen species.

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Tsyganova, A.V., Seliverstova, E.V., Gorshkov, A.P. et al. Effect of Elevated Temperature on Premature Senescence in Nodules of Pea (Pisum sativum L.) sym26 and sym27 Mutants. II. Ultrastructural Organization. Russ J Plant Physiol 70, 201 (2023). https://doi.org/10.1134/S1021443723603476

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