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A new method to prepare macroporous copolymer of glycidyl methacrilate and ethylene dimethacrilate as enzyme carrier

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Abstract

Macroporous copolymer of glycidyl methacrylate and ethylene dimethacrylate containing surface epoxy groups was firstly synthesized with dodecyl alcohol and cyclohexanol as liquid pore-forming agents and nanosize calcium carbonate as solid porogenic agent. The scanning electron microscopy was used to characterize their surface structure. Under the optimum conditions, β-galactosidase from Aspergillus oryzae was immobilized on the support obtained above, and the basic property and the kinetic data of the reaction on immobilized enzyme were determined. These data were compared with those obtained for the enzyme immobilized on the support prepared only with the liquid solution as pore-forming agent. Satisfactory results were obtained in enzyme activity, immobilization yield, pH stability, thermal stability, operational stability, and Michaelis constants K M. The results indicated that the copolymer of glycidyl methacrilate and ethylene dimethacrylate newly prepared was more suitable to immobilize enzyme than the carrier synthesized with traditional method.

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Authors and Affiliations

  1. College of Chemistry and Environmental Science, Hebei University, Baoding, China

    Sufang Sun, Lingyun Dong, **aobing Xu & Shigang Shen

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  1. Sufang Sun
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  2. Lingyun Dong
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  3. **aobing Xu
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  4. Shigang Shen
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Correspondence to Sufang Sun.

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Sun, S., Dong, L., Xu, X. et al. A new method to prepare macroporous copolymer of glycidyl methacrilate and ethylene dimethacrilate as enzyme carrier. Kinet Catal 52, 330–335 (2011). https://doi.org/10.1134/S0023158411020200

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  • DOI: https://doi.org/10.1134/S0023158411020200

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