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Myocardial Electrophysiological Response to Ischemia and Reperfusion Depends on the Age of Rats

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Abstract

Age is a major driver of the risk of acute myocardial infarction and malignant ventricular arrhythmias. However, age-related changes in the ischemic myocardium remain largely unclear. The present study aimed to evaluate electrophysiological responses of myocardium to experimental ischemia-reperfusion conditions in rats aged 3, 12, and 24 months. Epicardial map** (64 leads) was performed in a total of 34 anesthetized animals. In each lead, activation time (AT), end of repolarization time (RT), and activation-repolarization interval (ARI), were measured as dV/dt min during QRS, dV/dt max during T-wave, and RT – AT difference, respectively. Dispersion of repolarization (DOR) was determined as the RTmax – RTmin difference throughout all leads. Ischemia was induced by a 5-min ligation of the left anterior descending coronary artery. It was found that the 24-month-old rats had shorter baseline ATs and ARIs (p < 0.05), greater AT prolongation and less ARI shortening at ischemia (p < 0.05) and more complete recovery of the ARI at reperfusion (p < 0.001; all comparisons vs 3-month-old animals). At reperfusion, the absolute values of AT and DOR were lower in the old and middle-aged animals as compared to the young rats (p < 0.05); however, the absolute values of ARI durations did not differ between the groups. The data obtained suggest existence of age-related differences in the sensitivity of myocardium to ischemia-reperfusion conditions and that old animals are more susceptible to adverse changes regarding activation spread and more resilient regarding repolarization characteristics as compared to young adult animals.

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Funding

The study was supported by Russian Science Foundation (RSF 22-25-00843).

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Authors and Affiliations

  1. Institute of Physiology, Komi Science Center, Ural Branch of Russian Academy of Sciences, Syktyvkar, Russia

    E. V. Minnebaeva, A. V. Durkina, J. E. Azarov & O. G. Bernikova

  2. Department of Biochemistry and Physiology, Institute of Medicine, Pitirim Sorokin Syktyvkar State University, Syktyvkar, Russia

    J. E. Azarov

  3. Department of Fundamental Medicine and Public Health, Institute of Medicine, Pitirim Sorokin Syktyvkar State University, Syktyvkar, Russia

    E. V. Minnebaeva

Authors
  1. E. V. Minnebaeva
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  2. A. V. Durkina
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  4. O. G. Bernikova
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Contributions

E.V.M.: conducting experiments, data processing and analysis, writing a manuscript; A.V.D.: conceptualization, experimental design, conducting experiments, data analysis; J.E.A.: data analysis, writing and editing a manuscript; O.G.B.: conceptualization, experimental design, planning an experiment, conducting experiments, data analysis, writing and editing a manuscript.

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Correspondence to E. V. Minnebaeva.

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The authors declare that they have no conflicts of interest.

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Translated by A. Polyanovsky

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Minnebaeva, E.V., Durkina, A.V., Azarov, J.E. et al. Myocardial Electrophysiological Response to Ischemia and Reperfusion Depends on the Age of Rats. J Evol Biochem Phys 58 (Suppl 1), S63–S73 (2022). https://doi.org/10.1134/S0022093022070079

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  • DOI: https://doi.org/10.1134/S0022093022070079

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