Abstract
The changes in the contribution of ATP-sensitive potassium channels (KATP) to basal vascular tone maintenance and acetylcholine (ACh)-mediated dilation of pial arteries after global cerebral ischemia (2VO + hypo model) caused by a single cerebral ischemia–reperfusion were studied in Wistar rats. Ischemia was modeled by bilateral carotid artery clam** for 12 min paralleled by maintaining the mean arterial pressure at 45 ± 2 mm Hg. Sham-operated rats served as a control. Vascular examination was performed on days 7, 14 and 21 after ischemia. Changes in the contribution of KATP channels to the basal vascular tone were assessed using intravital microphotography by the number of vessels constricted in response to the effect of a KATP channel blocker glibenclamide (10 µM). Changes in the contribution of KATP channels to vascular dilation were assessed by comparing the number and degree of arterial dilation in response to ACh (10–7 M, 5 min) before and after glibenclamide application. It was found that ischemia reduces the role of KATP channels in basal vascular tone maintenance in pial arteries during 14 days after ischemic exposure. At the same time, the role of KATP channels in the implementation of ACh-mediated dilatory responses of pial arteries also decreases. By postischemic day 21, KATP channels are virtually no longer involved in the dilatory response to ACh.
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This work was state budget-funded. It was also supported by the Program of Basic Research for long-term Development and Ensuring the Competitiveness of the Society and State.
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Conceptualization and experimental design: O.P.G.; data collection: I.B.S.; data processing: O.P.G. and I.B.S.; writing and editing a manuscript: O.P.G. and I.B.S.
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Translated by A. Polyanovsky
Russian Text © The Author(s), 2021, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2021, Vol. 107, No. 9, pp. 1150–1161https://doi.org/10.31857/S0869813921090053.
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Gorshkova, O.P., Sokolova, I.B. Ischemia Changes the Contribution of KATP-Channels to Basal Tone and Dilation of Rat Pial Arteries. J Evol Biochem Phys 57, 1120–1129 (2021). https://doi.org/10.1134/S0022093021050136
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DOI: https://doi.org/10.1134/S0022093021050136