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Haloperidol, a Sigma-1 Receptor Antagonist, Inhibits Ca2+ Responses in Rat Peritoneal Macrophages

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Abstract

Sigma-1 receptors are ubiquitous multifunctional ligand-regulated molecular chaperons in the membrane of the endoplasmic reticulum, having a unique history, structure and pharmacological profile. Sigma-1 receptors modulate a wide range of cellular processes in normal and pathological conditions, inclu-ding Ca2+ signaling processes. Using the Fura-2AM fluorescent Ca2+ probe, we have shown that sigma-1 receptor antagonist neuroleptic haloperidol significantly suppressed the mobilization of Ca2+ from intracellular Ca2+ stores and the subsequent store-dependent Ca2+ entry into cells caused by endoplasmic Ca2+-ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as immunomodulators glutoxim and molixan in rat peritoneal macrophages. The results indicated the participation of sigma-1 receptors in the complex signaling cascade caused by glutoxim or molixan and leading to an increase in intracellular Ca2+ concentration in macrophages, as well as the involvement of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.

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REFERENCES

  1. T.-P. Su, T.-C. Su, Y. Nakamura, et al., Trends Pharmacol. Sci. 37, 262 (2016).

    Article  Google Scholar 

  2. C. G. Rousseaux and S. F. Greene, J. Recept. Signal Transduction 36, 327 (2016).

    Google Scholar 

  3. H. R. Schmidt and A. C. Kruse, Trends Pharmacol. Sci. 40, 636 (2019).

    Article  Google Scholar 

  4. R. Aishwarya, C. S. Abdullah, M. Morshed, et al., Front. Physiol. 12, 705575 (2021).

    Article  Google Scholar 

  5. B. Penke, L. Fulop, M. Szucs, et al., Curr. Neuropharmacol. 16, 97 (2018).

    Google Scholar 

  6. E. J. Cobos, J. M. Entrena, F. R. Nieto, et al., Curr. Neuropharmacol. 6, 344 (2008).

    Article  Google Scholar 

  7. T. Maurice and T. -P. Su, Pharmacol. Ther. 124, 195 (2009).

    Article  Google Scholar 

  8. U. B. Chu and A. E. Ruoho, Mol. Pharmacol. 89, 142 (2016).

    Article  Google Scholar 

  9. S. W. Tam and L. Cook, Proc. Natl. Acad. Sci. U. S. A. 81, 5618 (1984).

    Article  ADS  Google Scholar 

  10. I. Pontisso and L. Combettes, Genes 12, 139 (2021).

    Article  Google Scholar 

  11. T. Hayashi and T. -P. Su, Cell 131, 596 (2007).

    Article  Google Scholar 

  12. S. Srivats, D. Balasuriya, M. Pasche, et al., J. Cell Biol. 213, 65 (2016).

    Article  Google Scholar 

  13. G. C. Brailoiu, E. Deliu, L. M. Console-Bram, et al., Biochem. J. 473, 1 (2016).

    Article  Google Scholar 

  14. S. Berlansky, C. Humer, M. Sallinger, et al., Int. J. Mol. Sci. 22, 471 (2021).

    Article  Google Scholar 

  15. L. S. Milenina, Z. I. Krutetskaya, V. G. Antonov, et al., Biophysics 66, 77 (2021).

    Article  Google Scholar 

  16. L. S. Milenina, Z. I. Krutetskaya, V. G. Antonov, et al., Cell Tissue Biol. 16, 233 (2022).

    Article  Google Scholar 

  17. E. J. Cobos, E. Del Pozo, and J. M. Baeyens, J. Neurochem. 102, 812 (2007).

    Article  Google Scholar 

  18. G. Ayano, Res. Rev. J. Chem. 5, 53 (2016).

    Google Scholar 

  19. C. Randriamampita and A. Trautmann, Cell. Biol. 105, 761 (1987).

    Article  Google Scholar 

  20. R. A. Monahan, H. F. Dvorak, and A. M. Dvorak, Blood 58, 1089 (1981).

    Article  Google Scholar 

  21. Q. **e, Y. Zhang, C. Zhai, et al., J. Biol. Chem. 277, 16559 (2002).

    Article  Google Scholar 

  22. G. Grynkiewicz, M. Poenie, and R. Y. Tsien, J. Biol. Chem. 260, 3440 (1985).

    Article  Google Scholar 

  23. A. E. Borisov, L. A. Kozhemyakin, A. E. Antushevich, et al., Vestn. Khir. im. I. I. Grekova 4 (2), 32 (2001).

    Google Scholar 

  24. G. B. Sokolova, M. V. Sinitsyn, L. A. Kozhemyakin, et al., Antibiot. Khimioter. 47 (2), 20 (2002).

    Google Scholar 

  25. A. A. Antushevich, V. G. Antonov, A. N. Grebenyuk, et al., Vestn. Ross. Voen.-Med. Akad. 3 (43), 32 (2013).

    Google Scholar 

  26. O. A. Tolstoi, V. N. Tsygan, A. G. Klimov, et al., Izv. Ross. Voen.-Med. Akad. 38 (1), 271 (2019).

    Google Scholar 

  27. M. V. Dubina, V. V. Gomonova, A. E. Taraskina, et al., MedRxiv. Cited June 10, 2020.https://doi.org/10.1101/2020.09.25.20199562

  28. L. S. Kurilova, Z. I. Krutetskaya, O. E. Lebedev, et al., Cell Tissue Biol. 2, 322 (2008).

    Article  Google Scholar 

  29. L. S. Kurilova, Z. I. Krutetskaya, O. E. Lebedev, et al., Cell Tissue Biol. 6, 240 (2012).

    Article  Google Scholar 

  30. J. L. Harper, Y. Shin, and J. W. Daly, Proc. Natl. Acad. Sci. U. S. A. 94, 14912 (1997).

    Article  ADS  Google Scholar 

  31. J. L. Harper and J. W. Daly, Drug Dev. Res. 47, 107 (1999).

    Article  Google Scholar 

  32. S.-Y. Choi, Y.-H. Kim, Y.-K. Lee, et al., Br. J. Pharmacol. 132, 411 (2001).

    Article  Google Scholar 

  33. L. Wang, L. Zhang, S. Li et al., Sci. Rep. 5, 1 (2015).

    Google Scholar 

  34. M. S. Amer, L. McKeown, S. Tumova, et al., Br. J. Pharmacol. 168, 1445 (2013).

    Article  Google Scholar 

  35. G. Gasparre, C. Abate, R. Carlucci, et al., Pharmacol. Rep. 69, 542 (2017).

    Article  Google Scholar 

  36. S. F. Flaim, M. D. Brannan, S. C. Swioart et, al., Proc. Natl. Acad. Sci. U. S. A. 82, 1237 (1985).

    Article  ADS  Google Scholar 

  37. J. Church and E. J. Fletcher, Brit. J. Pharmacol. 116, 2801 (1995).

    Article  Google Scholar 

  38. B. Tarabova, M. Novakova, and L. Lacinova, Gen. Physiol. Biophys. 28, 249 (2009).

    Article  Google Scholar 

  39. H. Zhang and J. Cuevas, J. Neurophysiol. 87, 2867 (2002).

    Article  Google Scholar 

  40. C. M. Santi, F. S. Cayabyab, K. G. Sutton, et al., J. Neurosci. 22, 396 (2002).

    Article  Google Scholar 

  41. S.-Y. Tsai, T. Hayashi, T. Mori, et al., Cent. Nerv. Syst. Agents Med. Chem. 9, 184 (2009).

    Article  Google Scholar 

  42. M. V. Voronin, Y. V. Vakhitova, and S. B. Seredenin, Int. J. Mol. Sci. 21, 7088 (2020).

    Article  Google Scholar 

  43. J. M. Vela, Front. Pharmacol. 11, 582310 (2020).

    Article  Google Scholar 

  44. K. Hashimoto, Eur. Arch. Psychiatry Clin. Neurosci. 271, 249 (2021).

    Article  Google Scholar 

  45. D. E. Gordon, G. M. Jang, M. Bouhaddou, et al., Nature 583, 459 (2020).

    Article  ADS  Google Scholar 

  46. M. Plaze, D. Attali, A.-C. Petit, et al., L’Encephale 46, 169 (2020).

    Article  Google Scholar 

  47. P. Pandey, K. Prasad, A. Prakash, et al., J. Mol. Med. 98, 1659 (2020).

    Article  Google Scholar 

  48. B. L. Le, G. Andreoletti, T. Oskotsky, et al., Sci. Rep. 11, 12310 (2021).

    Article  ADS  Google Scholar 

  49. N. Hoertel, M. Sanchez-Rico, R. Vernet, et al., PLoS One 16, e0247122 (2021).

    Article  Google Scholar 

  50. N. Hoertel, M. Sanchez-Rico, R. Vernet, et al., Clin. Drug Invest. 41, 221 (2021).

    Article  Google Scholar 

  51. Y. Zhoua, T. K. Freyb, and J. J. Yanga, Cell Calcium 46, 1 (2009).

    Article  Google Scholar 

  52. X. Chen, R. Cao, and W. Zhong, Cells 9, 94 (2019).

    Article  Google Scholar 

  53. I. Solaimanzadeh, Cureus 12, e8069 (2020).

    Google Scholar 

  54. L.-K. Zhang, Y. Sun, H. Zeng, et al., Cell Discovery 6, 96 (2020).

    Article  Google Scholar 

  55. J. Miller, C. Bruen, M. Schnaus, et al., Crit. Care 24, 502 (2020).

    Article  Google Scholar 

  56. S. Berlansky, M. Sallinger, H. Grabmayr, et al., Cells 11, 253 (2022).

    Article  Google Scholar 

Download references

Funding

The work was carried out within the framework of the contracts of St. Petersburg State University for the performance of research works, projects no. 01/18-R&D dated 05.03.2018 and no. 05/03-20 dated March 12, 2020.

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Authors and Affiliations

  1. Saint-Petersburg State University, 199034, Saint-Petersburg, Russia

    L. S. Milenina, Z. I. Krutetskaya & N. I. Krutetskaya

  2. Saint-Petersburg State Pediatric Medical University, 194100, Saint-Petersburg, Russia

    V. G. Antonov

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  1. L. S. Milenina
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  2. Z. I. Krutetskaya
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  3. V. G. Antonov
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  4. N. I. Krutetskaya
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Correspondence to L. S. Milenina.

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CONFLICT OF INTEREST

The authors of this article declare that there is no conflict of interest.

COMPLIANCE WITH ETHICAL STANDARDS

The animals were kept and all manipulations with them were carried out in accordance with regulatory documents and the requirements of the Order of the Ministry of Health of the Russian Federation No. 267 dated 06/19/03 “On approval of the rules of laboratory practice in the Russian Federation.”

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Translated by E. Puchkov

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Abbreviations: CPA, cyclopiazonic acid; [Ca2+]i, intracellular concentration of Ca2+.

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Milenina, L.S., Krutetskaya, Z.I., Antonov, V.G. et al. Haloperidol, a Sigma-1 Receptor Antagonist, Inhibits Ca2+ Responses in Rat Peritoneal Macrophages. BIOPHYSICS 68, 552–560 (2023). https://doi.org/10.1134/S0006350923040140

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