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Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis

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Abstract

Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. We explored the protective effect of GP on human cardiomyocytes (HCMs) via the circ_0010729/miR-370-3p/RUNX1 axis. Overexpression of circ_0010729 abolished the effects of GP on HMC, such as suppression of apoptosis and increase in cell viability and proliferation. Overexpression of miR-370-3p reversed the effect of circ_0010729 overexpression, resulting in the stimulation of HMC viability and proliferation and inhibition of apoptosis. The knockdown of miR-370-3p suppressed the effects of GP in HCMs. RUNX1 silencing counteracted the effect of miR-370-3p knockdown and maintained GP-induced suppression of apoptosis and stimulation of HMC viability and proliferation. The levels of RUNX1 mRNA and protein were reduced in cells expressing miR-370-3p. In conclusion, this study confirmed that GP alleviated the I/R injury of myocardial cell via the circ_0010729/miR-370-3p/RUNX1 axis.

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Funding

This work was supported by ongoing institutional funding. No additional grants to carry out or direct this particular research were obtained.

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Authors and Affiliations

  1. Department of Cardiovascular Medicine, Shaoxing Central Hospital, 312000, Shaoxing City, Zhejiang Province, China

    Hailiang Ma, Yuanben Lu, Dewen Zhu, Zhenhua Jiang & Li Wang

  2. Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, Jiangxi, China

    FanZhi Zhang

  3. Department of Cardiovascular Medicine, First People’s Hospital of **aoshan District, 311200, Hangzhou, China

    Jun Peng

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  1. Hailiang Ma
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  3. Dewen Zhu
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  4. Zhenhua Jiang
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  6. Jun Peng
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  7. Li Wang
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Contributions

All authors contributed to the study design, interpretation of results, analysis of data, and review of the manuscript. H. Ma and Y. Lu conducted the experiments and wrote the manuscript; D. Zhu, Zh. Jiang, and F. Zhang analyzed the data, J. Peng and L. Wang reviewed the text of the article. All authors have read and approved the manuscript.

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Correspondence to Jun Peng or Li Wang.

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Ma, H., Lu, Y., Zhu, D. et al. Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis. Biochemistry Moscow 89, 973–986 (2024). https://doi.org/10.1134/S000629792405016X

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