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Generation of Membrane Potential by Cytochrome bd

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Abstract

An overview of current notions on the mechanism of generation of a transmembrane electric potential difference (Δψ) during the catalytic cycle of a bd-type triheme terminal quinol oxidase is presented in this work. It is suggested that the main contribution to Δψ formation is made by the movement of H+ across the membrane along the intra-protein hydrophilic proton-conducting pathway from the cytoplasm to the active site for oxygen reduction of this bacterial enzyme.

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Abbreviations

Δψ:

transmembrane electrical potential difference

Δp :

protonmotive force

τ:

time constant, reciprocal of rate constant (t1/e)

H+/e– :

proton/electron stoichiometry which in the case of Escherichia coli respiratory chain means number of protons released into the periplasm per electron used to reduce oxygen to water

RuBpy:

tris(2,2′-bipyridyl)ruthenium(II) chloride

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Acknowledgments

The author would like to express his deepest gratitude to M. I. Verkhovsky (passed away), I. N. Belevich, N. P. Belevich, D. A. Bloch (passed away), and A. Jasaitis for wonderful time spent measuring electrogenic activity of the enigmatic cytochrome bd.

Funding

This work was financially supported by the Russian Science Foundation (project no. 22-24-00045, https://rscf.ru/en/project/22-24-00045/).

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  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    Vitaliy B. Borisov

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Borisov, V.B. Generation of Membrane Potential by Cytochrome bd. Biochemistry Moscow 88, 1504–1512 (2023). https://doi.org/10.1134/S0006297923100073

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