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Molecular cloning of a cotton phosphatase gene and its functional characterization

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Abstract

In this study, we report isolation of a phosphatase gene designated GhHL1 from cotton and its functional characterization. GhHL1 transcripts were detected in all cotton tissues examined. Southern blotting analysis indicated that it exists in multiple-copies. Biochemical analysis showed that GhHL1 was Mg2+-dependent and cation-sensitive. Purified recombinant GhHL1 protein dephosphorylated both 3′,5′-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3′,5′-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities. Overexpression of GhHL1 complemented yeast hal2 mutant and enhanced yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response. Taken together, these results show that GhHL1 is a functional and good candidate gene, which might be used to improve salt tolerance in plants.

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Abbreviations

FDD-PCR:

fluorescence differential display-polymerase chain reaction

IP2:

inositol 1,4-bisphosphate

IP3:

inositol 1,4,5-trisphosphate

IPP1ase:

inositol polyphosphate 1-phosphatase

PAP:

3′(2′),5′-bisphosphate nucleotide

PAPase:

3′(2′),5′-bisphosphate nucleotidase

PAPS:

3′(2′),5′-bisphosphate nucleotide sulfate

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, Tsinghua University, Bei**g, 100084, China

    Shi-You Lu, Guang-Rong Zhao, Ai-Min Wu, Shu** Zhang & **-Yuan Liu

  2. Department of Horticulture and Landscape Architecture, Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN, 47907, USA

    M. A. Jenks

Authors
  1. Shi-You Lu
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  2. Guang-Rong Zhao
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  3. Ai-Min Wu
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  4. M. A. Jenks
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  5. Shu** Zhang
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  6. **-Yuan Liu
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Correspondence to **-Yuan Liu.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 1, pp. 105–116.

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Lu, SY., Zhao, GR., Wu, AM. et al. Molecular cloning of a cotton phosphatase gene and its functional characterization. Biochemistry Moscow 75, 85–94 (2010). https://doi.org/10.1134/S0006297910010116

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  • DOI: https://doi.org/10.1134/S0006297910010116

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